{"id":"W2054372701","doi":"10.1107/s0907444906009796","title":"Mutation of Gly51 to serine in the P-loop of<i>Lactobacillus casei</i>folylpolyglutamate synthetase abolishes activity by altering the conformation of two adjacent loops","year":2006,"lang":"en","type":"article","venue":"Acta Crystallographica Section D Biological Crystallography","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Lactobacillus casei; Mutant; Serine; Biochemistry; Enzyme; Biology; Alanine; Mutagenesis; Active site; ATPase; Stereochemistry; Chemistry; Amino acid; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001210861,0.0003358836,0.0005296585,0.0004602108,0.0001863527,0.00004483805,0.0004776832,0.0002177159,0.00007857427],"category_scores_gemma":[0.0001123734,0.0001959222,0.0003191001,0.0019514,0.0006205484,0.0003676155,0.0001036072,0.0002767148,2.335108e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002536866,"about_ca_system_score_gemma":0.00002460732,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002015029,"about_ca_topic_score_gemma":0.0007035077,"domain_scores_codex":[0.9971816,0.0005240217,0.0009525227,0.0004316223,0.0004937668,0.0004164655],"domain_scores_gemma":[0.9980353,0.0004036015,0.0007791814,0.0004899115,0.0002269201,0.00006505896],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004421377,0.0002531201,0.006269201,0.00007462392,0.00003034768,0.000003144722,0.0002613087,0.0008382353,0.9908503,0.0002718194,0.0002284494,0.0004772574],"study_design_scores_gemma":[0.00361443,0.003561917,0.4007127,0.0002413397,0.0002909347,0.0002648448,0.003276637,0.0005462192,0.5680243,0.005289335,0.01287965,0.001297637],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9953482,0.000210656,0.002348059,0.0002797198,0.00030709,0.0008128668,0.0002789105,0.00006541571,0.0003491431],"genre_scores_gemma":[0.9992852,0.0001159568,0.000201644,0.0001261121,0.00007786493,0.00008286632,0.0000935993,0.00001386996,0.000002934529],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.422826,"threshold_uncertainty_score":0.7989475,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01040918664341863,"score_gpt":0.2284287267627338,"score_spread":0.2180195401193152,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}