{"id":"W2054993171","doi":"10.1089/10430340050016148","title":"High-Efficiency Retroviral Transduction of Mammalian Cells on Positively Charged Surfaces","year":2000,"lang":"en","type":"article","venue":"Human Gene Therapy","topic":"Virus-based gene therapy research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"BC Cancer Agency","funders":"National Heart, Lung, and Blood Institute; U.S. Public Health Service; Dr. Mildred Scheel Stiftung für Krebsforschung","keywords":"Transduction (biophysics); Haematopoiesis; Biology; Cell culture; CD34; Retrovirus; Genetic enhancement; Transfection; Cord blood; Viral vector; Molecular biology; Green fluorescent protein; Cell biology; CD40; Stem cell; Immunology; In vitro; Cytotoxic T cell; Gene; Recombinant DNA; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007112385,0.0006436206,0.0005004752,0.0003118588,0.0002337921,0.0006624499,0.0005178324,0.0004264092,0.001176966],"category_scores_gemma":[0.0002810492,0.0002190701,0.0004215181,0.0003166799,0.0003780444,0.0002705398,0.0004368217,0.0008944912,0.0008600955],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002606162,"about_ca_system_score_gemma":0.0002022108,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002783938,"about_ca_topic_score_gemma":0.0004177624,"domain_scores_codex":[0.9992654,0.0001962496,0.00007099386,0.0001013348,0.0002860382,0.0000800107],"domain_scores_gemma":[0.999804,0.00005420278,0.00003684258,0.00005070128,0.00003409371,0.00002021778],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001993354,0.00001022705,0.00003925198,0.00002464125,0.000002975069,0.00002284568,0.00001821556,0.00004242399,0.998625,0.000182949,0.00002771157,0.0009838099],"study_design_scores_gemma":[0.000008775342,0.0001048545,0.000495537,0.000005253045,0.00001027483,0.0001360227,0.00001052187,0.0007676242,0.9948003,0.00006364984,0.003592179,0.00000498682],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8044088,0.003344254,0.1818901,0.000186717,0.0002379408,0.0007421693,0.001519114,0.0006607902,0.007010141],"genre_scores_gemma":[0.8630062,0.003093219,0.1200818,0.0001281871,0.00007117182,0.000484511,0.003133335,0.0002119164,0.0097896],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001176966,"threshold_uncertainty_score":0.003937304,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01922855154002279,"score_gpt":0.2781285158312504,"score_spread":0.2588999642912276,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}