{"id":"W2055078937","doi":"10.1128/aem.71.10.6247-6253.2005","title":"Chaperone-Mediated Folding and Maturation of the Penicillin Acylase Precursor in the Cytoplasm of <i>Escherichia coli</i>","year":2005,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada; National Science Council","keywords":"Chaperone (clinical); Periplasmic space; Cytoplasm; Escherichia coli; GroEL; Foldase; Chaperonin; Biology; Release factor; Protein folding; Inducer; Biochemistry; Overproduction; Signal peptide; Cell biology; Inclusion bodies; Chemistry; Recombinant DNA; Gene; RNA; Ribosome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001224506,0.00007240566,0.0001081965,0.00001308976,0.00004584175,0.000004312918,0.00007685083,0.00006675606,0.00004224947],"category_scores_gemma":[0.000003163045,0.00004199962,0.00001302878,0.00003299079,0.0002804936,0.00003376213,0.00004991544,0.00006464043,0.000004187605],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001101559,"about_ca_system_score_gemma":0.000003562201,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000008101606,"about_ca_topic_score_gemma":0.00002591802,"domain_scores_codex":[0.9995524,0.00004051975,0.0001535973,0.0001296626,0.00002873715,0.00009507275],"domain_scores_gemma":[0.9997597,0.00005352466,0.00008608265,0.00008971414,0.000001028271,0.000009930416],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004417541,0.00002068489,0.0007555143,0.000007469742,0.000002202587,6.435439e-8,0.0006641204,0.000023494,0.9979118,0.0002368468,0.00003561947,0.0002980761],"study_design_scores_gemma":[0.0005203774,0.00005387825,0.02456601,0.000005964403,0.00001380163,0.00001439705,0.0005213594,0.00002926178,0.9726196,0.00007914427,0.001509955,0.00006626169],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9991999,0.0002194893,0.00001659878,0.0001379546,0.0000514978,0.0001832372,0.00004292181,0.000002831153,0.0001455616],"genre_scores_gemma":[0.9993479,0.00008398845,0.0001782191,0.0003209147,0.00002231725,0.000009256343,0.00002213858,0.000003670159,0.00001162944],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02529214,"threshold_uncertainty_score":0.1712695,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003853108635740492,"score_gpt":0.1684420360820459,"score_spread":0.1645889274463055,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}