{"id":"W2055078937","doi":"10.1128/aem.71.10.6247-6253.2005","title":"Chaperone-Mediated Folding and Maturation of the Penicillin Acylase Precursor in the Cytoplasm of <i>Escherichia coli</i>","year":2005,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada; National Science Council","keywords":"Chaperone (clinical); Periplasmic space; Cytoplasm; Escherichia coli; GroEL; Foldase; Chaperonin; Biology; Release factor; Protein folding; Inducer; Biochemistry; Overproduction; Signal peptide; Cell biology; Inclusion bodies; Chemistry; Recombinant DNA; Gene; RNA; Ribosome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001415615,0.0002908899,0.0002212294,0.00006001719,0.00007779332,0.0001775574,0.0001289239,0.0001291583,0.0002435229],"category_scores_gemma":[0.0001137441,0.00007448907,0.0001754353,0.0001186114,0.00009402003,0.000133323,0.0001318755,0.0002495911,0.0001825878],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001833236,"about_ca_system_score_gemma":0.0002156494,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006599347,"about_ca_topic_score_gemma":0.000676146,"domain_scores_codex":[0.9999135,0.00002304101,0.000009207894,0.00001758011,0.00001760149,0.00001910309],"domain_scores_gemma":[0.9999377,0.00001065769,0.00001505338,0.000008480044,0.00001589087,0.00001228743],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004822703,0.0000091364,0.0001084917,0.00001988207,0.000001759018,0.00002850434,0.000007157686,0.00003773953,0.9991637,0.00003342575,0.00001502855,0.0005268786],"study_design_scores_gemma":[0.000005775692,0.00009829624,0.001078262,0.00000325774,0.000005154732,0.00008383374,0.00001438532,0.0007577898,0.9971155,0.00001405838,0.000820842,0.000002786411],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.993301,0.0005817681,0.004717747,0.00006412768,0.00002184912,0.00003019394,0.0002943909,0.00006678736,0.0009220956],"genre_scores_gemma":[0.9880916,0.0007720988,0.008266977,0.00005555783,0.000006917974,0.00003373644,0.001059031,0.00004376862,0.00167022],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006599347,"threshold_uncertainty_score":0.001330137,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003853108635740492,"score_gpt":0.1684420360820459,"score_spread":0.1645889274463055,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}