{"id":"W2055309995","doi":"10.1021/bi1000596","title":"Modulating the pH−Activity Profile of Cellulase by Substitution: Replacing the General Base Catalyst Aspartate with Cysteinesulfinate in Cellulase A from <i>Cellulomonas fimi</i>","year":2010,"lang":"en","type":"article","venue":"Biochemistry","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"","keywords":"Cellulase; Glycoside hydrolase; Chemistry; Active site; Enzyme; Biochemistry; Hydrolase; Stereochemistry; Site-directed mutagenesis; Mutagenesis; Catalysis; Residue (chemistry); Mutant","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002296595,0.0004342692,0.0002258839,0.0001164555,0.0001016693,0.0003976833,0.0003297485,0.0003344849,0.000608471],"category_scores_gemma":[0.0002265629,0.0001518576,0.0002462685,0.000186883,0.0002010578,0.0001905988,0.0002279422,0.0003703776,0.0002115488],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002642708,"about_ca_system_score_gemma":0.0002117836,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007027652,"about_ca_topic_score_gemma":0.0007549044,"domain_scores_codex":[0.9998204,0.00002873705,0.00003232158,0.00004183134,0.00004567184,0.00003113575],"domain_scores_gemma":[0.9998378,0.00002610049,0.00006869227,0.00002131188,0.00001910099,0.00002702584],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004960582,0.00001800382,0.0001188829,0.000009334258,0.000002768633,0.000016048,0.000004930973,0.00004435972,0.9993273,0.00001560844,0.000004400126,0.000388829],"study_design_scores_gemma":[0.000003144493,0.00007688281,0.0007742391,8.994375e-7,0.000004467658,0.00006869355,0.000006053977,0.0002456112,0.9985222,0.000005634447,0.0002898429,0.000002361648],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9964325,0.0001742387,0.002737188,0.00005389064,0.00001330548,0.00002647625,0.0001779736,0.00003840058,0.0003459327],"genre_scores_gemma":[0.9957879,0.000188595,0.003288506,0.00002921708,0.000003179105,0.00001375818,0.0002195168,0.00002337456,0.0004458559],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007027652,"threshold_uncertainty_score":0.002035558,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005313527694834118,"score_gpt":0.2051011583878862,"score_spread":0.1997876306930521,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}