{"id":"W2055309995","doi":"10.1021/bi1000596","title":"Modulating the pH−Activity Profile of Cellulase by Substitution: Replacing the General Base Catalyst Aspartate with Cysteinesulfinate in Cellulase A from <i>Cellulomonas fimi</i>","year":2010,"lang":"en","type":"article","venue":"Biochemistry","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"","keywords":"Cellulase; Glycoside hydrolase; Chemistry; Active site; Enzyme; Biochemistry; Hydrolase; Stereochemistry; Site-directed mutagenesis; Mutagenesis; Catalysis; Residue (chemistry); Mutant","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003315184,0.000239532,0.0001993898,0.00001812182,0.0001552906,0.00003790999,0.0003177017,0.0001786266,0.00002517576],"category_scores_gemma":[0.0001208289,0.0001535656,0.00009570938,0.0002210631,0.0002613186,0.00001211969,0.0001103101,0.0002509019,0.000002824419],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001758595,"about_ca_system_score_gemma":0.0001250458,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004358995,"about_ca_topic_score_gemma":0.0001601702,"domain_scores_codex":[0.9986317,0.00006407554,0.000326124,0.0005239676,0.0002048846,0.0002492434],"domain_scores_gemma":[0.9985532,0.00003937099,0.0002865724,0.0009414982,0.0001117484,0.00006764589],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001225753,0.0001069651,0.001909579,0.00003633559,0.0000326102,0.000006004745,0.0000519911,0.0003192427,0.9967188,0.000008903773,0.000559408,0.0001275574],"study_design_scores_gemma":[0.0006054097,0.00004074852,0.0003166874,0.00002812,0.00004218102,0.00001164961,0.00007389101,0.004613725,0.9933548,0.000004952591,0.0006987375,0.0002090825],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9977747,0.0005153072,0.0007038084,0.0001122331,0.0000853429,0.0002456184,0.0002069501,0.0000113935,0.0003446017],"genre_scores_gemma":[0.9968464,0.00001592188,0.0001681766,0.00005417275,0.0001988786,0.00006460494,0.001679348,0.00002556146,0.0009469415],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004294482,"threshold_uncertainty_score":0.6262225,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005313527694834118,"score_gpt":0.2051011583878862,"score_spread":0.1997876306930521,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}