{"id":"W2055359240","doi":"10.1093/oxfordjournals.jbchem.a003314","title":"Functional Tolerance of Streptomyces Subtilisin Inhibitor toward Conformational and Stability Changes Caused by Single-Point Mutations in the Hydrophobic Core","year":2002,"lang":"en","type":"article","venue":"The Journal of Biochemistry","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institute for Biological Sciences","funders":"","keywords":"Serine protease; Steric effects; Subtilisin; Chemistry; Side chain; Serine; Stereochemistry; Protease inhibitor (pharmacology); Amino acid; Conformational change; Protein structure; Biochemistry; Hydrophobic effect; Biophysics; Enzyme; Protease; Crystallography; Organic chemistry; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001381315,0.0002958257,0.000164723,0.0001329516,0.00009705893,0.0001963303,0.0001404241,0.0001607433,0.0006747674],"category_scores_gemma":[0.0001948999,0.00009653925,0.0001581036,0.0001263818,0.0002253916,0.00008871336,0.00009852831,0.0002752091,0.000200393],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001927061,"about_ca_system_score_gemma":0.000112759,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004141082,"about_ca_topic_score_gemma":0.0002883647,"domain_scores_codex":[0.9998742,0.00003242032,0.00001516432,0.00001892774,0.00003408226,0.00002513811],"domain_scores_gemma":[0.9997734,0.0000437542,0.00007807716,0.00002783027,0.00001750424,0.00005936365],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000146276,0.00001608017,0.0001610902,0.000008984813,0.000004589868,0.00004821465,0.000007891847,0.0001138224,0.9988708,0.00003869477,0.000005928757,0.0005776305],"study_design_scores_gemma":[0.0000119449,0.0003238879,0.003449821,0.000002215916,0.000009819445,0.0003246718,0.00001535691,0.001091241,0.9944093,0.0000262804,0.0003312443,0.000004173738],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.999005,0.0001053609,0.0005402769,0.00001093777,0.000004282624,0.000003350211,0.00005296186,0.00002192916,0.0002559389],"genre_scores_gemma":[0.9992633,0.00004537522,0.0003202293,0.000006821024,0.000001796243,0.000002916791,0.0001084046,0.000009578504,0.000241551],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006747674,"threshold_uncertainty_score":0.002257288,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02712928169616045,"score_gpt":0.2239380941423992,"score_spread":0.1968088124462387,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}