{"id":"W2056200903","doi":"10.1016/j.pep.2006.08.017","title":"Chemical ligation and cleavage on solid support facilitate recombinant peptide purification","year":2006,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Chemical Synthesis and Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"","keywords":"Recombinant DNA; Native chemical ligation; Peptide; Ligation; Cleavage (geology); Chemistry; Combinatorial chemistry; Chemical ligation; Yield (engineering); Chemical synthesis; Biochemistry; Chromatography; In vitro; Biology; Molecular biology; Materials science","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008062914,0.001122341,0.001481513,0.0006710914,0.000873547,0.001307538,0.0009440375,0.0009279308,0.009820828],"category_scores_gemma":[0.001458305,0.0009038015,0.0008723983,0.001043814,0.0004728078,0.0008295549,0.0009187077,0.00212323,0.01589988],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003563647,"about_ca_system_score_gemma":0.0008027924,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004296543,"about_ca_topic_score_gemma":0.001273022,"domain_scores_codex":[0.9992678,0.00009546287,0.00006838788,0.0001299032,0.0002785624,0.0001600247],"domain_scores_gemma":[0.9987473,0.00049102,0.0001351918,0.0002690545,0.0001973318,0.0001601259],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001042512,0.0001139597,0.000198724,0.0001291721,0.000011506,0.0001612271,0.00004118668,0.00009729934,0.9898913,0.0007278236,0.00117977,0.007343776],"study_design_scores_gemma":[0.00003766361,0.0000758802,0.0009669104,0.00001643516,0.00002130461,0.000402529,0.00002220348,0.0007155245,0.9718332,0.0005614835,0.02533056,0.00001616601],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.3464263,0.003748695,0.6027728,0.002518357,0.0009074226,0.00233125,0.006713926,0.009320394,0.02526073],"genre_scores_gemma":[0.3629076,0.006150884,0.5350953,0.001707597,0.0003359378,0.001869876,0.03025663,0.00333844,0.05833771],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.009820828,"threshold_uncertainty_score":0.0328539,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01104740418205831,"score_gpt":0.2318017976236976,"score_spread":0.2207543934416393,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}