{"id":"W2056200903","doi":"10.1016/j.pep.2006.08.017","title":"Chemical ligation and cleavage on solid support facilitate recombinant peptide purification","year":2006,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Chemical Synthesis and Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"","keywords":"Recombinant DNA; Native chemical ligation; Peptide; Ligation; Cleavage (geology); Chemistry; Combinatorial chemistry; Chemical ligation; Yield (engineering); Chemical synthesis; Biochemistry; Chromatography; In vitro; Biology; Molecular biology; Materials science","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001978551,0.0001415733,0.0001274794,0.0000391578,0.0001159696,0.00004828105,0.00007365726,0.0001517736,0.00001956218],"category_scores_gemma":[0.00007284802,0.0001212252,0.00004830581,0.00005833575,0.00006611732,0.00001100036,0.00003505137,0.00007792761,0.00001225287],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001114952,"about_ca_system_score_gemma":0.00001122382,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002625043,"about_ca_topic_score_gemma":0.000001545549,"domain_scores_codex":[0.9989595,0.00005428814,0.0002633967,0.0004398088,0.0001347469,0.0001482006],"domain_scores_gemma":[0.9994878,0.00001251128,0.0001278956,0.0002508746,0.00004600755,0.00007495808],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006367769,0.00008709158,0.0002256435,0.00002290336,0.000005013742,2.417321e-7,0.00002340704,0.000002737012,0.986083,0.0002036902,0.00112592,0.01215667],"study_design_scores_gemma":[0.0002598501,0.0000591505,0.001493301,0.00003879118,0.00001092754,0.000002348577,0.00002687624,0.0001638552,0.9830451,0.0008219075,0.01391902,0.000158848],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9965858,0.0002767368,0.001065788,0.0006537756,0.00001842552,0.0002969232,0.00001152188,0.00002196529,0.001069024],"genre_scores_gemma":[0.9967845,0.0001656547,0.0009090819,0.00004795714,0.0001002109,0.0001163716,0.0003907564,0.00001323181,0.001472207],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0127931,"threshold_uncertainty_score":0.4943419,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01104740418205831,"score_gpt":0.2318017976236976,"score_spread":0.2207543934416393,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}