{"id":"W2056616736","doi":"10.1074/jbc.m400782200","title":"Amino Acid Substitutions in the C-terminal AAA+ Module of Hsp104 Prevent Substrate Recognition by Disrupting Oligomerization and Cause High Temperature Inactivation","year":2004,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Heat shock proteins research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":50,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Heat shock protein; CLPB; Biochemistry; Chaperone (clinical); Amino acid; Heat shock; Chemical chaperone; Cytosol; Yeast; Protein folding; Cell biology; Chemistry; Biology; Mutant; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003806305,0.00010697,0.0001489189,0.00002577957,0.00006089438,0.00002224184,0.0001438407,0.0002341341,0.00000913067],"category_scores_gemma":[0.0003349693,0.00007044255,0.00005092839,0.0001377527,0.0001291672,0.0000151032,0.00003606797,0.0002857436,3.023546e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004447727,"about_ca_system_score_gemma":0.00008408979,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001189312,"about_ca_topic_score_gemma":0.000002739224,"domain_scores_codex":[0.9990928,0.00008537884,0.0003555099,0.000155074,0.0001594841,0.0001517677],"domain_scores_gemma":[0.9994741,0.00001964394,0.0001994895,0.0001106838,0.000140568,0.00005553324],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001024937,0.0001754438,0.001266039,0.00003849773,0.00001672971,0.00001738338,0.00003409914,0.00004717056,0.9976692,0.000005800511,0.00001003206,0.0006170653],"study_design_scores_gemma":[0.0005258783,0.0002790204,0.008220789,0.00006792054,0.000008850699,0.0002514543,0.0001455672,0.000003128352,0.9901745,0.000200216,0.00004068086,0.00008205118],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9989637,0.0003547912,0.0002137762,0.0002550799,0.00001608736,0.0001247811,0.00003180227,0.000001985357,0.0000380414],"genre_scores_gemma":[0.998886,0.0004839947,0.0002897552,0.00003090242,0.0001235037,0.00001084861,0.0001601105,0.000004888027,0.00000995288],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.007494803,"threshold_uncertainty_score":0.2872564,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02374170329430036,"score_gpt":0.2854670159391262,"score_spread":0.2617253126448258,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}