{"id":"W2058090291","doi":"10.1111/j.1574-6968.2002.tb11158.x","title":"PCR cloning of polyhydroxyalkanoate biosynthesis genes from<i>Burkholderia caryophylli</i>and their functional expression in recombinant<i>Escherichia coli</i>","year":2002,"lang":"en","type":"article","venue":"FEMS Microbiology Letters","topic":"biodegradable polymer synthesis and properties","field":"Materials Science","cited_by":30,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Chinese Academy of Sciences; Tsinghua University; National Natural Science Foundation of China; Public Health Agency of Canada","keywords":"Polyhydroxyalkanoates; Burkholderia; Escherichia coli; Cloning (programming); Pseudomonas; Biology; Gene; Microbiology; Recombinant DNA; Polyhydroxybutyrate; ATP synthase; Open reading frame; Biochemistry; Bacteria; Molecular biology; Chemistry; Genetics; Peptide sequence","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003303205,0.000664846,0.0004664619,0.0003513687,0.0001930708,0.0004372242,0.0004409134,0.0003282461,0.001245636],"category_scores_gemma":[0.0005239592,0.0003572221,0.0005478839,0.0005550701,0.0002715994,0.0002621366,0.0003373977,0.0008415727,0.001397067],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003693235,"about_ca_system_score_gemma":0.0004226441,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001682392,"about_ca_topic_score_gemma":0.001389938,"domain_scores_codex":[0.9995173,0.00007313809,0.00005759302,0.0001171175,0.0001292132,0.0001056962],"domain_scores_gemma":[0.9996656,0.00009918405,0.00007948637,0.00004166292,0.00007181214,0.00004221022],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003885043,0.00001836982,0.00009236684,0.00004989941,0.000002291136,0.00003490117,0.00001952362,0.00004506768,0.9979793,0.00007334025,0.00003258208,0.001613462],"study_design_scores_gemma":[0.00001679502,0.000141902,0.002795866,0.00001494455,0.00002621685,0.0002610674,0.000038429,0.0007309038,0.9915373,0.00004475222,0.004380317,0.00001143628],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8620366,0.002452997,0.1202635,0.0005228127,0.000244494,0.001297226,0.007818388,0.0007635315,0.004600405],"genre_scores_gemma":[0.7877794,0.003673485,0.1490748,0.0002651371,0.00007780053,0.000957838,0.03428311,0.0003884436,0.0235001],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001682392,"threshold_uncertainty_score":0.00416708,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02116797451327943,"score_gpt":0.1797827988879381,"score_spread":0.1586148243746586,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}