{"id":"W2058090291","doi":"10.1111/j.1574-6968.2002.tb11158.x","title":"PCR cloning of polyhydroxyalkanoate biosynthesis genes from<i>Burkholderia caryophylli</i>and their functional expression in recombinant<i>Escherichia coli</i>","year":2002,"lang":"en","type":"article","venue":"FEMS Microbiology Letters","topic":"biodegradable polymer synthesis and properties","field":"Materials Science","cited_by":30,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Chinese Academy of Sciences; Tsinghua University; National Natural Science Foundation of China; Public Health Agency of Canada","keywords":"Polyhydroxyalkanoates; Burkholderia; Escherichia coli; Cloning (programming); Pseudomonas; Biology; Gene; Microbiology; Recombinant DNA; Polyhydroxybutyrate; ATP synthase; Open reading frame; Biochemistry; Bacteria; Molecular biology; Chemistry; Genetics; Peptide sequence","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002693437,0.0003032647,0.0005110219,0.0001282037,0.0001691208,0.0000388937,0.0002720867,0.0002037263,0.0006875086],"category_scores_gemma":[0.00002317626,0.0002227976,0.00009228106,0.0001352684,0.0003959993,0.0001466935,0.0001304155,0.0001600732,0.00006702798],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002908256,"about_ca_system_score_gemma":0.00001342737,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007717874,"about_ca_topic_score_gemma":0.00004904758,"domain_scores_codex":[0.9981591,0.0003265333,0.0004798608,0.0005360885,0.00005805796,0.0004403886],"domain_scores_gemma":[0.9991354,0.000250882,0.0002191821,0.0003123336,0.00002064327,0.00006151861],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008877651,0.00006308006,0.00217745,0.00001807118,0.00002059659,0.000003960189,0.0007020248,0.000004972513,0.9930946,0.000002687704,0.001544759,0.002279005],"study_design_scores_gemma":[0.0003831818,0.0000456635,0.001602906,0.0001260776,0.00001359241,0.00001170255,0.000112079,0.0000667954,0.9933124,0.00001771783,0.004031618,0.0002762659],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9768606,0.02004303,0.00009147776,0.001682139,0.0009670816,0.0001259152,0.0001315019,0.00004358438,0.00005469418],"genre_scores_gemma":[0.9969698,0.0004287651,0.001054996,0.001291159,0.00009268816,0.00002697286,0.0000201147,0.00002831113,0.00008720001],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02010922,"threshold_uncertainty_score":0.9085421,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02116797451327943,"score_gpt":0.1797827988879381,"score_spread":0.1586148243746586,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}