{"id":"W2058497317","doi":"10.1016/s0022-1759(00)00249-0","title":"Flow cytometric analysis of intracellular IFN-γ, IL-4 and IL-10 in CD3+4+ T-cells from rat spleen","year":2000,"lang":"en","type":"article","venue":"Journal of Immunological Methods","topic":"Immune Cell Function and Interaction","field":"Immunology and Microbiology","cited_by":63,"is_retracted":false,"has_abstract":false,"ca_institutions":"Ottawa Hospital; University of Ottawa; Health Canada","funders":"","keywords":"Ionomycin; Cytokine; Molecular biology; Biology; Flow cytometry; Brefeldin A; Splenocyte; Intracellular; CD3; Antigen; Immunology; Cell biology; CD8; Golgi apparatus","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002076537,0.0008816541,0.0004736545,0.0006539714,0.0004153172,0.0004094439,0.0003535815,0.0005876595,0.004058592],"category_scores_gemma":[0.00009364405,0.0001824245,0.0005344276,0.0004480366,0.0003132761,0.0004029425,0.0002721232,0.002033592,0.0008812189],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002376847,"about_ca_system_score_gemma":0.0002534868,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005496186,"about_ca_topic_score_gemma":0.0006351328,"domain_scores_codex":[0.9998031,0.00001750319,0.00002311338,0.00004362237,0.00003318337,0.0000794641],"domain_scores_gemma":[0.9997959,0.00007099166,0.00002379359,0.00002267245,0.00003173636,0.00005492207],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004130744,0.00005703349,0.0002824298,0.00006072266,0.000006653721,0.00005080361,0.00002271608,0.00005914898,0.9981646,0.00007861176,0.00002693318,0.0007772667],"study_design_scores_gemma":[0.0001319646,0.0006288753,0.01005202,0.00001562733,0.00007971455,0.0002403854,0.0001105951,0.001784828,0.9856456,0.0002877272,0.001010246,0.00001248353],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9810303,0.001342522,0.009106233,0.0001990099,0.0002008868,0.0001543309,0.002430869,0.0002724203,0.005263355],"genre_scores_gemma":[0.9704319,0.001819383,0.01385524,0.000260705,0.0002221037,0.0004465406,0.002709356,0.00007672977,0.01017806],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004058592,"threshold_uncertainty_score":0.01357734,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02130969966908296,"score_gpt":0.3042436996608618,"score_spread":0.2829339999917789,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}