{"id":"W2058584763","doi":"10.1006/prep.2001.1582","title":"Secretory Production and Purification of Functional Full-Length Streptavidin from Bacillus subtilis","year":2002,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":34,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"","keywords":"Streptavidin; Biotinylation; Bacillus subtilis; Mutant; Biotin; Chemistry; Biochemistry; Biology; Molecular biology; Gene; Bacteria; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003624385,0.0004765804,0.0005125619,0.0002805793,0.0002917481,0.0005552042,0.0003619179,0.0003191294,0.0006734597],"category_scores_gemma":[0.0004005551,0.0002880737,0.0004378383,0.0006326712,0.0002978176,0.000388605,0.0004163214,0.0009212761,0.0009947651],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007471986,"about_ca_system_score_gemma":0.0005538323,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002797133,"about_ca_topic_score_gemma":0.002649195,"domain_scores_codex":[0.9997784,0.0000325148,0.00003419672,0.00003332548,0.00007118567,0.00005037649],"domain_scores_gemma":[0.9996861,0.00005452548,0.00004248945,0.00004924854,0.00007833645,0.00008932585],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001350072,0.00002877632,0.0001425452,0.00006984825,0.000006188044,0.00005538503,0.00005334328,0.00007492595,0.9975348,0.0001850824,0.0001125432,0.001601596],"study_design_scores_gemma":[0.00003276811,0.0001410029,0.002664736,0.00001164696,0.00001699196,0.0003209685,0.00007533191,0.0008658656,0.9906994,0.0001318095,0.005028492,0.00001113091],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9728066,0.002153134,0.01954523,0.0004522801,0.0001288819,0.00009864678,0.002882774,0.0002429152,0.001689572],"genre_scores_gemma":[0.9541876,0.001800444,0.0217074,0.0001521559,0.00003215302,0.00008188463,0.01166035,0.0001762739,0.01020155],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002797133,"threshold_uncertainty_score":0.005561709,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01767391086306012,"score_gpt":0.2126330323083393,"score_spread":0.1949591214452792,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}