{"id":"W2058584763","doi":"10.1006/prep.2001.1582","title":"Secretory Production and Purification of Functional Full-Length Streptavidin from Bacillus subtilis","year":2002,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Biotin and Related Studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":34,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"","keywords":"Streptavidin; Biotinylation; Bacillus subtilis; Mutant; Biotin; Chemistry; Biochemistry; Biology; Molecular biology; Gene; Bacteria; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001371834,0.000124438,0.0001082586,0.00004432929,0.0001484304,0.00001521851,0.00005126713,0.0002036341,0.00008196508],"category_scores_gemma":[0.0001163993,0.0001059091,0.00003026813,0.00006262849,0.0001290075,0.00001321835,0.00003998434,0.0001009061,0.000004988274],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006943224,"about_ca_system_score_gemma":0.000008728518,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001142463,"about_ca_topic_score_gemma":0.00000403134,"domain_scores_codex":[0.9990596,0.0000842022,0.0002223303,0.000398788,0.0001338257,0.0001013236],"domain_scores_gemma":[0.9994333,0.000008526677,0.0001606987,0.0002419962,0.0001010684,0.00005441964],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008237505,0.00007092106,0.0005379974,0.00003282759,0.00002227102,9.710507e-8,0.0002275819,0.000004391168,0.9835556,0.000058103,0.001424911,0.01398292],"study_design_scores_gemma":[0.0003573759,0.0001189434,0.01061986,0.00008089456,0.00002162839,0.000004358121,0.0002876969,0.0003102169,0.9757747,0.0001225821,0.01213516,0.0001665706],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9863998,0.0110357,0.0008856107,0.0006722998,0.000137526,0.0004143168,0.00002713844,0.00002122191,0.0004063837],"genre_scores_gemma":[0.9941924,0.003047842,0.001054174,0.00001668953,0.0001781982,0.00006663162,0.0001491275,0.00001115274,0.001283814],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01381635,"threshold_uncertainty_score":0.4318848,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01767391086306012,"score_gpt":0.2126330323083393,"score_spread":0.1949591214452792,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}