{"id":"W2058605910","doi":"10.3791/1569","title":"Extraction of High Molecular Weight Genomic DNA from Soils and Sediments","year":2009,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Microbial Community Ecology and Physiology","field":"Environmental Science","cited_by":21,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia; University of British Columbia Hospital","funders":"British Columbia Knowledge Development Fund; Genome British Columbia; Tula Foundation","keywords":"genomic DNA; Metagenomics; DNA extraction; DNA; Lysis; Chemistry; Chromatography; Isoamyl alcohol; Biology; Computational biology; Biochemistry; Polymerase chain reaction; Gene; Alcohol","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.000171794,0.0001068628,0.0002715756,0.00004527744,0.00006755835,0.000006925445,0.0001874411,0.0000925103,0.002305032],"category_scores_gemma":[0.0000127938,0.00009640191,0.00006352669,0.00005427141,0.00009015154,0.0001679526,0.00007518996,0.0001566008,0.00002497115],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00007475558,"about_ca_system_score_gemma":0.0000102845,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001573758,"about_ca_topic_score_gemma":0.000003222911,"domain_scores_codex":[0.9990959,0.0001578426,0.0003724645,0.0001050539,0.0001334558,0.0001353058],"domain_scores_gemma":[0.9993334,0.00004406379,0.0003975531,0.0001353482,0.00001267013,0.00007698929],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002678398,0.0003012134,0.000725129,0.000001091135,0.00005088456,0.00001135236,0.0005043174,0.0000329935,0.9965741,0.00006199337,0.0007545289,0.0007145391],"study_design_scores_gemma":[0.001912045,0.0006316822,0.07633899,0.00002038627,0.00003960895,0.00002877204,0.0000799492,0.00002639126,0.9138882,0.005813744,0.001099424,0.0001207794],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9987621,0.0003022205,0.0002192297,0.00006768043,0.0001991696,0.00006765409,0.000003552223,0.00000364332,0.0003747351],"genre_scores_gemma":[0.9974334,0.0001064539,0.002001363,0.0003832294,0.00003419816,6.290942e-7,0.000006497753,0.000005927385,0.00002826797],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0826859,"threshold_uncertainty_score":0.998607,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0126554403844046,"score_gpt":0.3477512579484755,"score_spread":0.3350958175640709,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}