{"id":"W2058682134","doi":"10.1016/j.ab.2008.07.027","title":"High-efficiency transfection of nucleic acids by the modified calcium phosphate precipitation method in chondrocytes","year":2008,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":false,"ca_institutions":"Hôpital Notre-Dame; Université de Montréal","funders":"Canadian Arthritis Network; Canadian Institutes of Health Research","keywords":"Transfection; Gene knockdown; Nucleic acid; Oligonucleotide; Chemistry; Gene silencing; Molecular biology; Cell biology; RNA interference; Calcium; Small interfering RNA; Extracellular matrix; Cartilage; RNA; Gene; Biochemistry; Biology; Anatomy","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007287352,0.000535376,0.0007151422,0.0004166603,0.0003836831,0.0007018884,0.0006245831,0.0005815962,0.001212866],"category_scores_gemma":[0.0002913687,0.0003721366,0.0005363235,0.0004678825,0.0004754728,0.000411885,0.0003870307,0.001182303,0.001099096],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005383246,"about_ca_system_score_gemma":0.0006474985,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002881288,"about_ca_topic_score_gemma":0.006058199,"domain_scores_codex":[0.9990876,0.0001260717,0.0001086499,0.0001509592,0.0003787866,0.0001479864],"domain_scores_gemma":[0.9997597,0.00005655007,0.00003902496,0.00005675067,0.00005239102,0.00003557878],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004309641,0.00001499694,0.00003074779,0.00002492037,0.000002492746,0.00001903192,0.00001947783,0.00002904295,0.9988084,0.0001136384,0.00003822309,0.0008559435],"study_design_scores_gemma":[0.0000109916,0.00005784461,0.0004859944,0.000002550917,0.000009208606,0.0000491466,0.000006531486,0.0004325893,0.9972146,0.00002413945,0.001702277,0.00000422847],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8375725,0.004917194,0.144441,0.0004878307,0.0004071219,0.0004997475,0.00207768,0.00122517,0.008371786],"genre_scores_gemma":[0.8917497,0.002464329,0.07318286,0.0001449193,0.00006306323,0.0003968991,0.002483704,0.0003029962,0.0292116],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002881288,"threshold_uncertainty_score":0.00572902,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01550995011272639,"score_gpt":0.2740972706071848,"score_spread":0.2585873204944584,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}