{"id":"W2058682134","doi":"10.1016/j.ab.2008.07.027","title":"High-efficiency transfection of nucleic acids by the modified calcium phosphate precipitation method in chondrocytes","year":2008,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":false,"ca_institutions":"Hôpital Notre-Dame; Université de Montréal","funders":"Canadian Arthritis Network; Canadian Institutes of Health Research","keywords":"Transfection; Gene knockdown; Nucleic acid; Oligonucleotide; Chemistry; Gene silencing; Molecular biology; Cell biology; RNA interference; Calcium; Small interfering RNA; Extracellular matrix; Cartilage; RNA; Gene; Biochemistry; Biology; Anatomy","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001715788,0.0001501076,0.0001773093,0.00002841791,0.00006060406,0.000008645696,0.0002235368,0.000198542,0.00003009725],"category_scores_gemma":[0.00008921555,0.0001185584,0.0001227766,0.0002178631,0.0001674745,0.000005656773,0.00003403987,0.0001315624,0.00000555211],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002116021,"about_ca_system_score_gemma":0.00006017844,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001283141,"about_ca_topic_score_gemma":0.000005524538,"domain_scores_codex":[0.9989361,0.00004969884,0.000303468,0.0003381073,0.0001589821,0.0002136584],"domain_scores_gemma":[0.999477,0.00003378122,0.00006335361,0.0002848733,0.00008437206,0.00005667608],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001242461,0.0001337415,0.000602508,0.000019404,0.00003678069,0.000002004595,0.00005868775,0.0003682403,0.9965531,0.0000398002,0.00122922,0.0008322843],"study_design_scores_gemma":[0.0004307985,0.0001625497,0.001088392,0.00001330371,0.00002550217,0.00001558958,0.00006908338,0.002574223,0.9953057,0.00003885672,0.0001372589,0.0001387526],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9806422,0.0005916759,0.01784566,0.0001548876,0.00005550846,0.00009673375,0.00002461928,0.000007636453,0.0005810921],"genre_scores_gemma":[0.998466,0.0002094526,0.0002232297,0.0001034161,0.00006279896,0.00001513316,0.0001136902,0.0000109344,0.0007953373],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01782382,"threshold_uncertainty_score":0.4834672,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01550995011272639,"score_gpt":0.2740972706071848,"score_spread":0.2585873204944584,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}