{"id":"W2059425107","doi":"10.1006/prep.2000.1285","title":"Purification and Characterization of a luxO Promoter Binding Protein LuxT from Vibrio harveyi","year":2000,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"bioluminescence and chemiluminescence research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"McGill University","funders":"Medical Research Council","keywords":"Vibrio harveyi; Operon; Affinity chromatography; Repressor; Biology; Autoinducer; Biochemistry; Chemistry; Molecular biology; Vibrio; Bacteria; Quorum sensing; Gene; Transcription factor; Escherichia coli; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001995943,0.0001434194,0.0001309377,0.00005192262,0.00009924598,0.00005302004,0.0001233511,0.0002015842,0.000118],"category_scores_gemma":[0.00003772858,0.0001280051,0.00002777868,0.0001084389,0.0001122037,0.00004153846,0.00003956756,0.00009093509,0.00001017643],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006901907,"about_ca_system_score_gemma":0.00003263404,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002636726,"about_ca_topic_score_gemma":0.000001558095,"domain_scores_codex":[0.9988611,0.00008783102,0.0002679399,0.0004391351,0.0001735317,0.0001704804],"domain_scores_gemma":[0.9993722,0.000004389803,0.0001385556,0.0003048669,0.00009190248,0.00008801762],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001283429,0.00006950708,0.001021743,0.00007085119,0.000006246887,1.976079e-7,0.0001290168,3.731442e-8,0.9617559,0.0000120059,0.00001760196,0.03678852],"study_design_scores_gemma":[0.0003291811,0.00008115527,0.006538461,0.0001958322,0.000005480058,0.000002015725,0.00004130309,0.00007545174,0.9888104,0.00003811757,0.003733639,0.0001490253],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9978523,0.0002599484,0.0004560701,0.0003837811,0.00001153953,0.0008690992,0.0000410962,0.00001522787,0.0001109457],"genre_scores_gemma":[0.9951598,0.0004562197,0.001120824,0.00001492078,0.0001056081,0.0002690498,0.0008354082,0.00001727251,0.002020914],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03663949,"threshold_uncertainty_score":0.5219898,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01268343430081697,"score_gpt":0.2452471130783406,"score_spread":0.2325636787775236,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}