{"id":"W2059510043","doi":"10.1007/s00253-013-5049-9","title":"A simple and rapid method to isolate purer M13 phage by isoelectric precipitation","year":2013,"lang":"en","type":"article","venue":"Applied Microbiology and Biotechnology","topic":"Bacteriophages and microbial interactions","field":"Environmental Science","cited_by":33,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"","keywords":"Isoelectric point; Polyethylene glycol; Bacteriophage; Chromatography; PEG ratio; Isoelectric focusing; Precipitation; Escherichia coli; Phage display; Yield (engineering); Chemistry; Biology; Biochemistry; Materials science; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008208346,0.002169698,0.001503788,0.00214915,0.001036588,0.0008934286,0.001682741,0.001185665,0.003674034],"category_scores_gemma":[0.001037346,0.001058677,0.001220562,0.001105652,0.0006938131,0.0009179557,0.001854939,0.003841307,0.00773701],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000351371,"about_ca_system_score_gemma":0.000728387,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009273691,"about_ca_topic_score_gemma":0.00157693,"domain_scores_codex":[0.9983079,0.0002604089,0.0001577512,0.0003371348,0.0007620165,0.0001747212],"domain_scores_gemma":[0.9994803,0.0001424399,0.00005281905,0.000123553,0.0001312299,0.00006982168],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007029617,0.0002099491,0.0005056767,0.0002643363,0.00003437977,0.0001009723,0.00007183298,0.00008297286,0.9591796,0.0002828765,0.002069525,0.03712746],"study_design_scores_gemma":[0.00009817032,0.0006436377,0.008611047,0.00006449658,0.0001649748,0.002095909,0.000124246,0.002040759,0.9414417,0.001162597,0.04337932,0.0001731284],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.06291464,0.006693264,0.9132134,0.001002301,0.001041407,0.002367572,0.002446169,0.004346011,0.005975187],"genre_scores_gemma":[0.1527467,0.00622526,0.7918802,0.001026897,0.0003636577,0.003291786,0.01567909,0.00087375,0.02791259],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003674034,"threshold_uncertainty_score":0.01229084,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003175678565288556,"score_gpt":0.2104043011144209,"score_spread":0.2072286225491324,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}