{"id":"W2059878343","doi":"10.1016/j.jim.2011.11.011","title":"Optimization of a whole blood intracellular cytokine assay for measuring innate cell responses to mycobacteria","year":2011,"lang":"en","type":"article","venue":"Journal of Immunological Methods","topic":"Immune Response and Inflammation","field":"Immunology and Microbiology","cited_by":14,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"National Institute of Allergy and Infectious Diseases; National Institutes of Health; Wellcome Trust; American Educational Research Association","keywords":"Innate immune system; Biology; CD14; Monocyte; CD11c; Cytokine; Immunology; Intracellular; Flow cytometry; Green fluorescent protein; Myeloid; TLR2; Cell biology; Immune system; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.005234743,0.0001925967,0.000603876,0.0003784937,0.000104458,0.0000142342,0.000389687,0.0003607376,0.0003016353],"category_scores_gemma":[0.003607627,0.0001434479,0.0002511996,0.0002410409,0.0001148336,0.0002056253,0.000103103,0.0003623633,0.00001512581],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003805938,"about_ca_system_score_gemma":0.00008932123,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005481161,"about_ca_topic_score_gemma":1.147837e-7,"domain_scores_codex":[0.9961185,0.00226705,0.00108773,0.000167713,0.00005957309,0.0002994299],"domain_scores_gemma":[0.9972593,0.0009483006,0.0009078424,0.0002500938,0.0006045098,0.00003000278],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0068487,0.0003054976,0.0000952495,0.00002694048,0.0002801075,0.000007498787,0.0006485873,0.0001255183,0.9746659,0.0001348889,0.00003458899,0.01682656],"study_design_scores_gemma":[0.001919111,0.002671751,0.003284367,0.00009170771,0.0001722156,0.000131071,0.0002298004,0.00001897793,0.984585,0.0002547377,0.006481602,0.0001596518],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.67027,0.003167387,0.3253376,0.0001241931,0.0006130281,0.0002566112,0.000008049386,0.00001821308,0.0002048594],"genre_scores_gemma":[0.5109662,0.0002435687,0.4882256,0.00004278626,0.00003757249,0.00001681235,0.000008424391,0.00001920355,0.0004398041],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.162888,"threshold_uncertainty_score":0.5849636,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05648657769317325,"score_gpt":0.3041723889255387,"score_spread":0.2476858112323654,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}