{"id":"W2060399876","doi":"10.1006/abio.2001.5417","title":"Frontal Affinity Chromatography Coupled to Mass Spectrometry for Screening Mixtures of Enzyme Inhibitors","year":2001,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":64,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"","keywords":"Chemistry; Chromatography; Mass spectrometry; Enzyme; Substrate (aquarium); Biotinylation; Enzyme assay; Enzyme kinetics; Reaction rate constant; Affinity chromatography; Stereochemistry; Active site; Kinetics; Organic chemistry; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000231628,0.0001919759,0.0002408643,0.0001177737,0.00007650692,0.00002886295,0.0002661379,0.0002434379,0.0001691378],"category_scores_gemma":[0.000449767,0.0001876908,0.0002737225,0.0004122209,0.0001256604,0.000003917954,0.0001004558,0.0001267281,0.00001071632],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001757201,"about_ca_system_score_gemma":0.00007267725,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003058467,"about_ca_topic_score_gemma":0.000007423253,"domain_scores_codex":[0.9985278,0.00002025563,0.0003139597,0.000443376,0.0002998961,0.0003947335],"domain_scores_gemma":[0.9990338,0.00003723123,0.0000712163,0.0003909833,0.0001871162,0.0002796357],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003634196,0.00009634377,0.003555222,0.00004761854,0.0001038786,0.000004944,0.000004893216,0.00002418507,0.9899427,0.00003501411,0.005507688,0.000314091],"study_design_scores_gemma":[0.0006967551,0.0002272271,0.0007519792,0.00002276742,0.00002446292,0.00001054244,0.00005672757,0.0009171961,0.9921797,0.00005471261,0.00483515,0.0002227863],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9630617,0.0003078509,0.03428473,0.000393608,0.00003745873,0.0002922481,0.00006489563,0.000018269,0.001539283],"genre_scores_gemma":[0.990025,0.00002850235,0.008395891,0.0001537941,0.000310005,0.00003120419,0.000216268,0.00002241974,0.0008168744],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02696338,"threshold_uncertainty_score":0.7653807,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01377006693623553,"score_gpt":0.2873384962195799,"score_spread":0.2735684292833443,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}