{"id":"W2060399876","doi":"10.1006/abio.2001.5417","title":"Frontal Affinity Chromatography Coupled to Mass Spectrometry for Screening Mixtures of Enzyme Inhibitors","year":2001,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":64,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"","keywords":"Chemistry; Chromatography; Mass spectrometry; Enzyme; Substrate (aquarium); Biotinylation; Enzyme assay; Enzyme kinetics; Reaction rate constant; Affinity chromatography; Stereochemistry; Active site; Kinetics; Organic chemistry; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001020394,0.002436587,0.002021834,0.001548425,0.0007594932,0.0009287427,0.001487118,0.001045801,0.004519576],"category_scores_gemma":[0.001185149,0.000978481,0.0007077897,0.001048221,0.0004304814,0.0007322716,0.0007738154,0.002004155,0.00401936],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001024447,"about_ca_system_score_gemma":0.001327035,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002710067,"about_ca_topic_score_gemma":0.005940684,"domain_scores_codex":[0.9988672,0.0001566073,0.00006075271,0.0002198359,0.0004866239,0.0002089922],"domain_scores_gemma":[0.9993582,0.0002347588,0.00005967416,0.00004847766,0.0001514223,0.0001473722],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003929129,0.000246756,0.000342742,0.0001263593,0.0000804139,0.00006248776,0.00001685667,0.0002012288,0.983896,0.0002850899,0.0009871863,0.01336188],"study_design_scores_gemma":[0.0001095204,0.0007115674,0.003203912,0.00003117945,0.0001842568,0.0009018285,0.00003750008,0.007069257,0.9781964,0.0004684929,0.009046418,0.00003975094],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2887454,0.02068087,0.6643338,0.001482855,0.0005157203,0.002133096,0.004655718,0.006103238,0.01134931],"genre_scores_gemma":[0.4747165,0.03464323,0.4269156,0.00487699,0.0005065926,0.003018012,0.02372947,0.0008243606,0.03076933],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004519576,"threshold_uncertainty_score":0.01511949,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01377006693623553,"score_gpt":0.2873384962195799,"score_spread":0.2735684292833443,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}