{"id":"W2060756795","doi":"10.1038/nprot.2007.15","title":"Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method","year":2007,"lang":"en","type":"article","venue":"Nature Protocols","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":417,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Manitoba","funders":"","keywords":"Transformation (genetics); Yeast; Saccharomyces cerevisiae; Transformation efficiency; Plasmid; Genomic library; PEG ratio; DNA; Protoplast; Computational biology; Molecular biology; Chemistry; Biology; Cell biology; Computer science; Biochemistry; Gene; Base sequence; Agrobacterium","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005958705,0.001092989,0.001205829,0.0008141532,0.000638494,0.0009594465,0.001036155,0.0004626426,0.003073099],"category_scores_gemma":[0.0003595054,0.0004671875,0.0007047729,0.0009023521,0.000554328,0.0004685049,0.0007944495,0.001856847,0.003906695],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005431962,"about_ca_system_score_gemma":0.0009707758,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001432994,"about_ca_topic_score_gemma":0.002307892,"domain_scores_codex":[0.99957,0.00005799505,0.00004537979,0.00009538161,0.0001769798,0.0000542834],"domain_scores_gemma":[0.9997522,0.00004886497,0.0000343421,0.0000781178,0.00003447428,0.0000520223],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008347967,0.00004131913,0.00005680714,0.00007676541,0.00001081476,0.00004613233,0.00003041166,0.0000902035,0.9929146,0.000474837,0.0005724747,0.005602247],"study_design_scores_gemma":[0.00002599319,0.00004001555,0.0002703838,0.00000508635,0.00001708264,0.00009691975,0.000007960128,0.0006404746,0.9898028,0.0001443571,0.008937772,0.0000111699],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2971044,0.002755048,0.6575171,0.00104213,0.0006056986,0.001730813,0.007351512,0.01564118,0.01625214],"genre_scores_gemma":[0.6224219,0.00424641,0.3072935,0.0002824399,0.0001171086,0.001511411,0.02445598,0.003258754,0.0364125],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003073099,"threshold_uncertainty_score":0.01028055,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01576373665039952,"score_gpt":0.3712872066783656,"score_spread":0.355523470027966,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}