{"id":"W2061011679","doi":"10.1186/1746-4811-4-5","title":"Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus","year":2008,"lang":"en","type":"article","venue":"Plant Methods","topic":"Plant Virus Research Studies","field":"Agricultural and Biological Sciences","cited_by":149,"is_retracted":false,"has_abstract":true,"ca_institutions":"Plant Biotechnology Institute","funders":"Yale University","keywords":"Nicotiana benthamiana; Tobacco rattle virus; Complementary DNA; Biology; Gene silencing; Phytoene desaturase; cDNA library; Rapid amplification of cDNA ends; Gene; Genetics; Molecular cloning","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009716424,0.0002640356,0.0004958511,0.00003827318,0.000953929,0.00009779505,0.0003979429,0.0001394068,0.0001672001],"category_scores_gemma":[0.0008050352,0.0001092036,0.0001729153,0.0003475783,0.000107294,0.0003121583,0.0002346505,0.0001932497,0.00001708491],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000465413,"about_ca_system_score_gemma":0.00004591462,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005570129,"about_ca_topic_score_gemma":0.00009028052,"domain_scores_codex":[0.9977733,0.000460809,0.0003403,0.000463223,0.0003016986,0.0006606102],"domain_scores_gemma":[0.996402,0.003127019,0.0001295286,0.00009666839,0.00007570288,0.0001690584],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000231007,0.00004811947,0.0000739808,0.00000752303,0.00005026087,0.00002663839,0.0003242418,0.00004965929,0.986854,0.00003027533,0.001445906,0.01085844],"study_design_scores_gemma":[0.0003285165,0.0001578525,0.001223059,0.00002540204,0.00002420313,0.00007432776,0.0002518667,0.002621188,0.9871191,0.0001951459,0.007638241,0.0003411108],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.9934173,0.0008710901,0.00260788,0.0001538429,0.0003257331,0.0005035042,0.001705724,0.0001529787,0.0002619651],"genre_scores_gemma":[0.1039645,0.002421594,0.8897907,0.0006955152,0.001566972,0.0002474211,0.0007559575,0.00001262365,0.0005446372],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.8894528,"threshold_uncertainty_score":0.7336947,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.2336801823257018,"score_gpt":0.3680978763606056,"score_spread":0.1344176940349038,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}