{"id":"W2061891609","doi":"10.1021/bi002018s","title":"Characterization of the Glu and Asp Residues in the Active Site of Human β-Hexosaminidase B","year":2001,"lang":"en","type":"article","venue":"Biochemistry","topic":"Studies on Chitinases and Chitosanases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"Hospital for Sick Children; University of Toronto; University of British Columbia","funders":"Canadian Institutes of Health Research","keywords":"Active site; Hexosaminidase; Chemistry; Residue (chemistry); Stereochemistry; Enzyme; Site-directed mutagenesis; Acetamide; Mutant; Biochemistry; Hydrolase","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00005736147,0.00008250119,0.00009269724,0.00000896035,0.00005269897,0.000004689231,0.0001263282,0.00006605505,0.000005410439],"category_scores_gemma":[0.00005177882,0.0000531564,0.00004577241,0.00008095449,0.000149121,0.000001630474,0.00008734864,0.00004593241,1.070795e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000031668,"about_ca_system_score_gemma":0.00001768539,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003458373,"about_ca_topic_score_gemma":0.00008019103,"domain_scores_codex":[0.9995086,0.0000292701,0.0001453152,0.0001447215,0.0000842747,0.00008785173],"domain_scores_gemma":[0.9995742,0.00001298124,0.0001158547,0.0002511927,0.00003220926,0.00001349888],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003441056,0.00005273982,0.03471692,0.00003054466,0.00001369765,9.401485e-7,0.000119247,1.562705e-7,0.9646332,0.000009154352,0.00006604638,0.0003228931],"study_design_scores_gemma":[0.0001672074,0.00004355216,0.2683015,0.00002403071,0.000008889811,0.000005933352,0.0003929191,6.516905e-7,0.7307723,0.000007880685,0.0002302531,0.0000449309],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9987023,0.0002800009,0.000001457123,0.0001553662,0.00001677731,0.00008094912,0.00006328064,0.000001598971,0.0006982795],"genre_scores_gemma":[0.9993948,0.0002196444,0.00000641057,0.00009155033,0.00008631835,0.000007950031,0.00008906887,0.000005366973,0.00009894519],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.233861,"threshold_uncertainty_score":0.2167655,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008657767866266408,"score_gpt":0.2436829315866184,"score_spread":0.235025163720352,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}