{"id":"W2062062954","doi":"10.1021/ja042642i","title":"Single-Molecule Tracing on a Fluidic Microchip for Quantitative Detection of Low-Abundance Nucleic Acids","year":2005,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Microfluidic and Bio-sensing Technologies","field":"Engineering","cited_by":117,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Division of Biological Infrastructure; McMaster University","keywords":"Chemistry; Molecular beacon; Fluorescence; Microfluidics; Confocal; Molecule; Microreactor; Nucleic acid; Analytical Chemistry (journal); Laser-induced fluorescence; DNA; Nanotechnology; Chromatography; Oligonucleotide; Optics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004355786,0.0004344495,0.0004692915,0.0005190941,0.0003189228,0.0004812495,0.0008087172,0.0004923834,0.0009390568],"category_scores_gemma":[0.00068738,0.0002728915,0.0002898155,0.000241067,0.0004090117,0.0004117692,0.0004047237,0.0006522099,0.0005638677],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004448131,"about_ca_system_score_gemma":0.0005246216,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008023385,"about_ca_topic_score_gemma":0.001466431,"domain_scores_codex":[0.9995227,0.00006188729,0.00002013855,0.0001113888,0.0002507726,0.00003313674],"domain_scores_gemma":[0.999605,0.0001891699,0.00006139106,0.00005906815,0.00004952246,0.000035873],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003335474,0.0000204887,0.0001554293,0.00006336122,0.00000606783,0.00003506896,0.00003503249,0.0006167424,0.979672,0.0009096001,0.0001869366,0.01826588],"study_design_scores_gemma":[0.000006400324,0.00006565455,0.000293831,0.000005849036,0.000009164737,0.0001077422,0.000005186721,0.01832064,0.9756395,0.0002215894,0.005312813,0.00001156271],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2453427,0.002315894,0.7452373,0.0004323783,0.0002361444,0.0002119736,0.0003638421,0.003194077,0.002665615],"genre_scores_gemma":[0.313208,0.001131583,0.6814905,0.0001182991,0.00004787362,0.0002841952,0.0001741306,0.00008829752,0.003457197],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.0009390568,"threshold_uncertainty_score":0.003227353,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01249769859555133,"score_gpt":0.2343517177754509,"score_spread":0.2218540191798996,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}