{"id":"W2062509850","doi":"10.1002/(sici)1097-4644(20000315)76:4<674::aid-jcb15>3.0.co;2-2","title":"Circular YAC vectors containing short mammalian origin sequences are maintained under selection as HeLa episomes","year":2000,"lang":"en","type":"article","venue":"Journal of Cellular Biochemistry","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; University of British Columbia","funders":"Office of Research Infrastructure Programs, National Institutes of Health","keywords":"Biology; Plasmid; Molecular biology; Transfection; Ethidium bromide; Origin of replication; Transformation (genetics); Extrachromosomal DNA; DNA; Cloning vector; Vector (molecular biology); Gene; Genetics; Recombinant DNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0003198567,0.0002609501,0.000283176,0.00006445211,0.00009430869,0.00005441913,0.0002666467,0.0002708842,0.0002749297],"category_scores_gemma":[0.00007199093,0.0002558356,0.0002534146,0.0001539609,0.00007406092,0.000009396189,0.0000285586,0.0002669985,0.000008988608],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000767754,"about_ca_system_score_gemma":0.0001827102,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001218307,"about_ca_topic_score_gemma":0.000003124343,"domain_scores_codex":[0.9985563,0.00004371946,0.0004637252,0.0002949485,0.0002982188,0.0003431528],"domain_scores_gemma":[0.9992134,0.00001447386,0.0001711283,0.0002332599,0.000158705,0.0002090294],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001095798,0.00004331764,0.002269834,0.00004626199,0.0001480493,0.00005908289,0.00002650928,0.002589408,0.9935217,0.000008180448,0.0003371819,0.0008408706],"study_design_scores_gemma":[0.000420841,0.0002858395,0.0004745624,0.00008346947,0.0000680158,0.0004459938,0.0003771406,0.00006498782,0.9915121,0.00009027303,0.0058746,0.0003021423],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9875008,0.002586132,0.008780375,0.000137886,0.0001800976,0.00007316736,0.00000533261,0.00001401718,0.0007221224],"genre_scores_gemma":[0.9966194,0.0002664125,0.0005399346,0.000113261,0.0009507775,0.00000358663,0.00004061027,0.0000375616,0.001428436],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00911855,"threshold_uncertainty_score":0.9999894,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007763339658855925,"score_gpt":0.2740691145844509,"score_spread":0.2663057749255949,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}