{"id":"W2062620374","doi":"10.1021/bp000090b","title":"Effect of Ammonia on the Glycosylation of Human Recombinant Erythropoietin in Culture","year":2000,"lang":"en","type":"article","venue":"Biotechnology Progress","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":84,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba","funders":"Natural Sciences and Engineering Research Council of Canada; University of Manitoba; Manitoba Health Research Council","keywords":"Exoglycosidase; Glycan; Oligosaccharide; Sialic acid; Chemistry; Biochemistry; Glycosylation; Recombinant DNA; PNGase F; Fucosylation; Chromatography; clone (Java method); High-performance liquid chromatography; Ion chromatography; Chinese hamster ovary cell; Ammonia; Cell culture; Glycoprotein; Biology; Gene; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001877294,0.0003430576,0.0004457374,0.0000778161,0.0001428664,0.0002807707,0.0002392309,0.0001872242,0.0008427331],"category_scores_gemma":[0.0002761634,0.0001192169,0.0001889887,0.0001032771,0.0001575486,0.000139289,0.0003611748,0.00033737,0.0002489138],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002556418,"about_ca_system_score_gemma":0.0002330361,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001878989,"about_ca_topic_score_gemma":0.002438082,"domain_scores_codex":[0.9997613,0.00007251882,0.00002061212,0.00003760904,0.00006224422,0.0000457234],"domain_scores_gemma":[0.9997994,0.00005390098,0.00003472639,0.0000226681,0.00004422562,0.00004507218],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005657047,0.0000459957,0.0002283227,0.00004112103,0.000008272205,0.0000806604,0.00002961362,0.00007024913,0.997295,0.00001464916,0.00005049169,0.001569989],"study_design_scores_gemma":[0.00002263583,0.0008804376,0.004851536,0.00001038067,0.00001500248,0.0001524527,0.00003223527,0.000683422,0.9912635,0.00001923089,0.002061284,0.000007757328],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9950528,0.001615256,0.0009356042,0.0000685957,0.00005021594,0.00004278937,0.000304067,0.00004598411,0.001884759],"genre_scores_gemma":[0.9923114,0.001216541,0.002544733,0.0001103126,0.00003105199,0.00004983634,0.0007701134,0.00003488406,0.002931024],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001878989,"threshold_uncertainty_score":0.003736079,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007012240768043751,"score_gpt":0.2920181490893743,"score_spread":0.2850059083213305,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}