{"id":"W2062620374","doi":"10.1021/bp000090b","title":"Effect of Ammonia on the Glycosylation of Human Recombinant Erythropoietin in Culture","year":2000,"lang":"en","type":"article","venue":"Biotechnology Progress","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":84,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Manitoba","funders":"Natural Sciences and Engineering Research Council of Canada; University of Manitoba; Manitoba Health Research Council","keywords":"Exoglycosidase; Glycan; Oligosaccharide; Sialic acid; Chemistry; Biochemistry; Glycosylation; Recombinant DNA; PNGase F; Fucosylation; Chromatography; clone (Java method); High-performance liquid chromatography; Ion chromatography; Chinese hamster ovary cell; Ammonia; Cell culture; Glycoprotein; Biology; Gene; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004369187,0.0001209391,0.0001756358,0.0000976859,0.00005653199,0.000005062897,0.0002859857,0.0004217516,0.00009227384],"category_scores_gemma":[0.00008463694,0.00007812698,0.00006492207,0.0002618162,0.0004244698,0.000002138228,0.00005984637,0.0002013932,0.00001225582],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001156137,"about_ca_system_score_gemma":0.00001726813,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003446771,"about_ca_topic_score_gemma":0.00004219652,"domain_scores_codex":[0.9990046,0.0001836935,0.0002370284,0.0002390503,0.0001438909,0.0001917243],"domain_scores_gemma":[0.999398,0.00001718518,0.00009558874,0.0004282469,0.00004206365,0.0000189092],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0008891738,0.000302177,0.01110055,0.00009083207,0.00004086784,0.000005949147,0.00006036457,0.00001336645,0.8260506,0.002196115,0.0005238022,0.1587262],"study_design_scores_gemma":[0.0007841252,0.003342771,0.002998332,0.00005068178,0.000004542257,0.000004306492,0.00001426558,0.00003296671,0.9836479,0.00009547197,0.008944349,0.0000802834],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9973689,0.0004731175,0.000004034643,0.0009891974,0.00002694177,0.000545565,0.000005131137,0.00001486624,0.0005722262],"genre_scores_gemma":[0.9992361,0.0001745316,0.00007513993,0.00001378574,0.00001502347,0.00005919626,0.00003022768,0.00001102551,0.0003849371],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1586459,"threshold_uncertainty_score":0.3252934,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007012240768043751,"score_gpt":0.2920181490893743,"score_spread":0.2850059083213305,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}