{"id":"W2062849321","doi":"10.1016/j.pep.2004.11.004","title":"Overexpression of human alanine:glyoxylate aminotransferase in Escherichia coli: renaturation from guanidine–HCl and affinity for pyridoxal phosphate co-factor","year":2004,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":22,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"University of British Columbia; Sick Kids Foundation","keywords":"Escherichia coli; Pyridoxal; Guanidine; Alanine; Pyridoxal 5-Phosphate; Chemistry; Biochemistry; Pyridoxal phosphate; Alanine aminotransferase; Glyoxylate cycle; Phosphate; Amino acid; Enzyme; Biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002002179,0.000168979,0.0002122202,0.00007476575,0.0001733628,0.00005934955,0.00008847852,0.0001791238,0.0000947379],"category_scores_gemma":[0.00007114794,0.0001406074,0.00002813891,0.00008982185,0.00006711634,0.0003822883,0.00002066132,0.000124835,0.000002176945],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003495566,"about_ca_system_score_gemma":0.00003690496,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000283862,"about_ca_topic_score_gemma":0.00003362371,"domain_scores_codex":[0.9988039,0.00007901517,0.0003804375,0.0003928285,0.0001833254,0.0001604534],"domain_scores_gemma":[0.9993914,0.00004597084,0.0002130632,0.0002033913,0.00007284401,0.00007326562],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003844148,0.00006245054,0.0003181551,0.00008202226,0.000002080356,3.250278e-7,0.0009747821,0.0000190893,0.9968517,0.0007326561,0.00002817934,0.0005441312],"study_design_scores_gemma":[0.001729056,0.0001483261,0.02340479,0.0002858429,0.000007773826,4.366917e-7,0.00006271998,0.0000755747,0.9697398,0.004257284,0.0001296381,0.0001587717],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9955519,0.0003243751,0.002733482,0.0001056151,0.00009139425,0.0009544009,0.0001292013,0.00004424028,0.00006532203],"genre_scores_gemma":[0.9948512,0.00004181841,0.004647691,0.00002811262,0.00007748319,0.0001400892,0.000163572,0.00001564346,0.0000343677],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02711193,"threshold_uncertainty_score":0.5733803,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01788646267478461,"score_gpt":0.2620271504160177,"score_spread":0.244140687741233,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}