{"id":"W2063136144","doi":"10.1016/j.pep.2011.03.009","title":"Development of a GMP Phase III purification process for VB4-845, an immunotoxin expressed in E. coli using high cell density fermentation","year":2011,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Toxin Mechanisms and Immunotoxins","field":"Immunology and Microbiology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"Viventia Bio (Canada)","funders":"","keywords":"Immunotoxin; Pseudomonas exotoxin; Chromatography; Escherichia coli; Chemistry; Recombinant DNA; Fermentation; Food science; Biochemistry; Cytotoxicity","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005062387,0.0002131593,0.0002821567,0.0001909269,0.0003375182,0.00002276394,0.0002088813,0.0002789822,0.0001219914],"category_scores_gemma":[0.00003922102,0.0002047887,0.00003674186,0.000147145,0.0001004042,0.0003954285,0.00002990649,0.0001496077,0.000005973155],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005009599,"about_ca_system_score_gemma":0.0001563465,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000957975,"about_ca_topic_score_gemma":0.000007219233,"domain_scores_codex":[0.9983549,0.0001761104,0.0006791212,0.0004582772,0.00006598586,0.0002656551],"domain_scores_gemma":[0.9988859,0.00001764834,0.000516811,0.0003389411,0.0002036795,0.00003703147],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001111645,0.0008966345,0.00005266665,0.0001223503,0.0000114264,2.14882e-7,0.0118318,0.000002931793,0.9790451,0.001077775,0.000003419354,0.00584407],"study_design_scores_gemma":[0.004874617,0.0001952646,0.002251618,0.0001595792,0.00001848992,0.000001515059,0.002709581,0.0001391738,0.9875478,0.001614876,0.0002491228,0.0002383206],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9538811,0.0004753069,0.04308898,0.00001094425,0.0001300616,0.002306841,0.00002867101,0.00004112276,0.00003696772],"genre_scores_gemma":[0.9677371,0.00001516328,0.03092412,0.00001023222,0.00001038327,0.000856972,0.0002725989,0.00002397498,0.0001494788],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01385597,"threshold_uncertainty_score":0.8351042,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04857647891897721,"score_gpt":0.2926696632867328,"score_spread":0.2440931843677556,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}