{"id":"W2063346783","doi":"10.3791/2597","title":"Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death","year":2011,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":536,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"University of Alberta; Natural Sciences and Engineering Research Council of Canada; Agriculture Funding Consortium","keywords":"Propidium iodide; Annexin; Apoptosis; Pi; Flow cytometry; Annexin A5; Cell biology; Programmed cell death; Biology; Viability assay; Molecular biology; Cell; Membrane permeability; Chemistry; Biochemistry; Membrane","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001572305,0.001721095,0.001705734,0.002531776,0.0006291203,0.0008202536,0.001849125,0.001633158,0.004148528],"category_scores_gemma":[0.001739076,0.0006071262,0.0008172679,0.001841713,0.0004755033,0.001065973,0.001000427,0.002943465,0.002045389],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005678396,"about_ca_system_score_gemma":0.0009069856,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000481373,"about_ca_topic_score_gemma":0.00112284,"domain_scores_codex":[0.9961005,0.0008344359,0.0004241396,0.0006781261,0.001715742,0.0002470062],"domain_scores_gemma":[0.9986331,0.0003255016,0.0002278393,0.0001589571,0.0005540618,0.0001005318],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002986171,0.0001976306,0.0006796069,0.0009062509,0.00007957644,0.0004116587,0.0001214578,0.0003824776,0.9643833,0.001229595,0.002331787,0.02897814],"study_design_scores_gemma":[0.00009635681,0.001103213,0.002412123,0.0001234878,0.000162016,0.002834566,0.00005770979,0.005095234,0.9258428,0.001344507,0.0608122,0.0001157956],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1276325,0.05284434,0.7882791,0.0009285499,0.002341029,0.002909108,0.003689276,0.00522608,0.01615007],"genre_scores_gemma":[0.2283314,0.03420714,0.6887919,0.001045526,0.0006177125,0.004478452,0.00835363,0.0003630257,0.03381116],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.004148528,"threshold_uncertainty_score":0.01387817,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.07392232736525504,"score_gpt":0.4215702206095357,"score_spread":0.3476478932442806,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}