{"id":"W2063525719","doi":"10.1186/s13104-015-1045-8","title":"Validation of reference genes for quantitative real-time PCR (qPCR) analysis of Actinobacillus suis","year":2015,"lang":"en","type":"article","venue":"BMC Research Notes","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":10,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Reference genes; Gene; Biology; Anoxic waters; Gene expression; Computational biology; Genetics; Ecology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.008110629,0.001477802,0.001124775,0.001644083,0.0007919095,0.001496985,0.001013793,0.0009890514,0.001030623],"category_scores_gemma":[0.008455783,0.0005108343,0.001037967,0.001692416,0.001091019,0.0006934028,0.0007296459,0.000958674,0.0009998702],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008958499,"about_ca_system_score_gemma":0.001280608,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001329525,"about_ca_topic_score_gemma":0.002370138,"domain_scores_codex":[0.9896059,0.002235265,0.001082015,0.001976958,0.004548643,0.0005511404],"domain_scores_gemma":[0.9943493,0.001559784,0.0007407583,0.0006883928,0.002562993,0.00009884909],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002405149,0.0001243095,0.003748682,0.0002949584,0.00002207829,0.00004800372,0.0001759672,0.0007764206,0.984443,0.0002875632,0.0002518989,0.009586724],"study_design_scores_gemma":[0.00001364999,0.0007054264,0.009222752,0.0001020213,0.00008427793,0.0001383097,0.0001673131,0.003121707,0.979818,0.0002456712,0.006346256,0.00003457137],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6830816,0.006494896,0.2929573,0.0005267967,0.0007992808,0.001218136,0.008640075,0.001571198,0.004710656],"genre_scores_gemma":[0.6102177,0.004263696,0.3574499,0.0005465538,0.0001648736,0.00381114,0.01876088,0.0006761543,0.00410914],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.008110629,"threshold_uncertainty_score":0.04289359,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.2722662016986199,"score_gpt":0.4698183699325985,"score_spread":0.1975521682339786,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}