{"id":"W2063853502","doi":"10.1242/dev.114488","title":"Tissue-specific genome editing in <i>Ciona</i> embryos by CRISPR/Cas9","year":2014,"lang":"en","type":"article","venue":"Development","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":156,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Heart, Lung, and Blood Institute; York University; American Heart Association; National Science Foundation","keywords":"CRISPR; Ciona; Biology; Ciona intestinalis; Cas9; Genome editing; Electroporation; Zebrafish; Gene; Genetics; Gene knockout; Genome; Computational biology; Cell biology","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004548084,0.0005882972,0.000373066,0.0004007452,0.0005579928,0.0006943985,0.0009434557,0.000555525,0.001676135],"category_scores_gemma":[0.0003279693,0.000368325,0.000359436,0.0001619468,0.0006813116,0.0003218149,0.0006722622,0.001133283,0.0007680671],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005113822,"about_ca_system_score_gemma":0.0003328561,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00220065,"about_ca_topic_score_gemma":0.004066778,"domain_scores_codex":[0.9996361,0.00004484825,0.00004466744,0.0001076338,0.0001121067,0.00005464081],"domain_scores_gemma":[0.9996803,0.00008412934,0.00007767302,0.00006910404,0.00002930442,0.000059482],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000008537886,0.000004660749,0.00005993474,0.0000131659,0.000001312508,0.00005443164,0.00001427016,0.00003616343,0.9989692,0.0001589629,0.00003565468,0.0006435958],"study_design_scores_gemma":[0.000007076859,0.00004604932,0.001666131,0.000007530756,0.000008239796,0.0003784276,0.00002144615,0.0006438271,0.9925477,0.00006852278,0.004595189,0.00000983991],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8751079,0.001443792,0.1053384,0.0003779616,0.0002471088,0.0004119778,0.001544069,0.001895924,0.01363287],"genre_scores_gemma":[0.9223799,0.001198692,0.06454213,0.0002733893,0.00002797334,0.0002303254,0.001377473,0.0004054355,0.009564624],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00220065,"threshold_uncertainty_score":0.005607247,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005497049869607241,"score_gpt":0.249811973623329,"score_spread":0.2443149237537217,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}