{"id":"W2064120308","doi":"10.1038/srep04407","title":"One-step antibody immobilization-based rapid and highly-sensitive sandwich ELISA procedure for potential in vitro diagnostics","year":2014,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":138,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada","funders":"","keywords":"Chromatography; Detection limit; Chemistry; Microtiter plate; Human serum albumin; Fetuin; Albumin; Ionic strength; In vitro; Biomarker; Phosphate buffered saline; Biochemistry; Glycoprotein","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007050833,0.0001535921,0.000204968,0.0001250681,0.0001780867,0.00009695003,0.00005859206,0.0001458958,5.354009e-7],"category_scores_gemma":[0.0004929658,0.0001442047,0.00008577359,0.000213556,0.0002325309,0.000006124683,0.00004527019,0.00005564692,2.840869e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001400618,"about_ca_system_score_gemma":0.00008819559,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001069712,"about_ca_topic_score_gemma":0.00002526897,"domain_scores_codex":[0.9983875,0.00005139338,0.0003491385,0.0007742976,0.0001878978,0.0002497613],"domain_scores_gemma":[0.9989862,0.00003378936,0.0002108125,0.0004319328,0.0002656958,0.00007152073],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006357352,0.00008831307,0.001000163,0.0000326548,0.00001495396,0.000009165093,0.000009183424,0.0000600571,0.9953965,0.000007794752,0.0009729473,0.002344709],"study_design_scores_gemma":[0.0002664065,0.0001001904,0.0007317967,0.00003672039,0.00004135387,0.00001974146,0.00002377433,0.003524384,0.9888242,0.0003271128,0.005897802,0.0002065016],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9421531,0.0002652947,0.0561275,0.000173007,0.0005743311,0.0005717814,0.00001922667,0.000043276,0.00007251035],"genre_scores_gemma":[0.9864336,0.00004692918,0.01202872,0.0001220212,0.0001224987,0.00002143949,0.001047182,0.00001753244,0.0001601376],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04428046,"threshold_uncertainty_score":0.5880497,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005879955767134014,"score_gpt":0.2582945709214396,"score_spread":0.2524146151543056,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}