{"id":"W2064120308","doi":"10.1038/srep04407","title":"One-step antibody immobilization-based rapid and highly-sensitive sandwich ELISA procedure for potential in vitro diagnostics","year":2014,"lang":"en","type":"article","venue":"Scientific Reports","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":138,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada","funders":"","keywords":"Chromatography; Detection limit; Chemistry; Microtiter plate; Human serum albumin; Fetuin; Albumin; Ionic strength; In vitro; Biomarker; Phosphate buffered saline; Biochemistry; Glycoprotein","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001050759,0.001705514,0.001083686,0.0005443998,0.0002423929,0.0004464356,0.001294262,0.001242724,0.0009328386],"category_scores_gemma":[0.000776646,0.0005411374,0.0007705263,0.0003077994,0.0002902308,0.0005074384,0.0005731081,0.001309874,0.001120697],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004237906,"about_ca_system_score_gemma":0.0003387083,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000277036,"about_ca_topic_score_gemma":0.0005790904,"domain_scores_codex":[0.9988675,0.0002948927,0.00007102903,0.0002122461,0.0004529626,0.0001013115],"domain_scores_gemma":[0.9994794,0.0001788216,0.00008615145,0.000049818,0.0001591984,0.00004669558],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002785681,0.00002731603,0.00008151832,0.0001232692,0.00001330141,0.00003066658,0.00001105646,0.00005760017,0.9958191,0.00007547328,0.00007388105,0.003658907],"study_design_scores_gemma":[0.00000788164,0.0002228795,0.0005251304,0.00001151112,0.00003551655,0.0004094039,0.000007737153,0.001797807,0.9943495,0.00004689629,0.002567236,0.00001859014],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.2487901,0.03436013,0.7096396,0.000551138,0.0008420248,0.0008002829,0.0006791725,0.001959162,0.00237846],"genre_scores_gemma":[0.4308684,0.01269581,0.5469574,0.0004821984,0.0001968922,0.0009529562,0.0012579,0.00006917301,0.006519234],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001705514,"threshold_uncertainty_score":0.005557001,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005879955767134014,"score_gpt":0.2582945709214396,"score_spread":0.2524146151543056,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}