{"id":"W2064450167","doi":"10.1021/ja412297x","title":"Thioamide-Based Fluorescent Protease Sensors","year":2014,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":56,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institute of Neurological Disorders and Stroke; National Institute of General Medical Sciences; National Institute of Allergy and Infectious Diseases; Queen's University; Alfred P. Sloan Foundation; Searle Scholars Program; University of Pennsylvania; National Institutes of Health; Kinship Foundation; National Science Foundation","keywords":"Chemistry; Proteases; Protease; Papain; Thermolysin; Subtilisin; Trypsin; Fluorescence; Thioamide; Chymotrypsin; Biochemistry; Serine protease; Enzyme; Proteolysis; Stereochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002484716,0.00009636609,0.0001577971,0.000005750531,0.00004655842,0.00001032156,0.0002345941,0.00005385408,0.000006860563],"category_scores_gemma":[0.00007112486,0.0000622209,0.0005393234,0.0000850905,0.0002296497,0.000001876668,0.00002857643,0.0001932217,0.000001155311],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002490065,"about_ca_system_score_gemma":0.0000595186,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007519839,"about_ca_topic_score_gemma":3.691885e-7,"domain_scores_codex":[0.9992664,0.00005296857,0.0002144727,0.0001098076,0.0002130998,0.000143222],"domain_scores_gemma":[0.9992878,0.00001208567,0.0003225278,0.000203567,0.00008908082,0.00008487394],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009155112,0.00005008653,0.004318499,0.000008805201,0.000034675,4.843673e-7,0.00002500836,0.000101991,0.9936957,0.000003421288,0.00109208,0.0005777342],"study_design_scores_gemma":[0.0004159503,0.0001300316,0.002492958,0.00001642753,0.00003774536,0.0000129287,0.00004207881,0.0004702829,0.9695693,0.00003136794,0.026683,0.00009791722],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9911781,0.00005096353,0.007397489,0.001186973,0.00007179649,0.00006324184,0.000002046223,0.000003513077,0.00004585184],"genre_scores_gemma":[0.9963424,0.0000258628,0.00189251,0.001279029,0.0003930403,0.000001333481,0.00002000926,0.00001224471,0.00003358222],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02559092,"threshold_uncertainty_score":0.2537295,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00534656311819397,"score_gpt":0.2142682229217387,"score_spread":0.2089216598035447,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}