{"id":"W2064450167","doi":"10.1021/ja412297x","title":"Thioamide-Based Fluorescent Protease Sensors","year":2014,"lang":"en","type":"article","venue":"Journal of the American Chemical Society","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":56,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Institute of Neurological Disorders and Stroke; National Institute of General Medical Sciences; National Institute of Allergy and Infectious Diseases; Queen's University; Alfred P. Sloan Foundation; Searle Scholars Program; University of Pennsylvania; National Institutes of Health; Kinship Foundation; National Science Foundation","keywords":"Chemistry; Proteases; Protease; Papain; Thermolysin; Subtilisin; Trypsin; Fluorescence; Thioamide; Chymotrypsin; Biochemistry; Serine protease; Enzyme; Proteolysis; Stereochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003995009,0.0005104372,0.0002835271,0.0002214299,0.0001349273,0.0004086586,0.0008040882,0.0006199917,0.001119227],"category_scores_gemma":[0.0005062577,0.0002146373,0.0002597713,0.0002022075,0.000359151,0.0005323536,0.0003230171,0.0007415126,0.0003430105],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004308416,"about_ca_system_score_gemma":0.0001661129,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004157503,"about_ca_topic_score_gemma":0.0007590096,"domain_scores_codex":[0.9996943,0.00005521451,0.00001332254,0.00007859847,0.000115699,0.00004279994],"domain_scores_gemma":[0.9997537,0.00006915038,0.00007400571,0.00002030272,0.00005242559,0.00003038834],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000534788,0.0000113308,0.00005970779,0.00003019896,0.000005581219,0.00002556969,0.00001264971,0.0001853812,0.997502,0.0002521552,0.00008105727,0.001780878],"study_design_scores_gemma":[0.000009559446,0.00007997091,0.0001785578,0.000002448536,0.000006623586,0.00008727976,0.000005918897,0.002047373,0.9963064,0.00004448319,0.001226553,0.000004898872],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8476563,0.004384904,0.1397746,0.0003484347,0.0001635758,0.0001232623,0.0005926152,0.001137547,0.005818726],"genre_scores_gemma":[0.9198745,0.002499891,0.07092666,0.0002628006,0.00006297345,0.0001753988,0.0006181509,0.00006166222,0.005518012],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001119227,"threshold_uncertainty_score":0.003744185,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00534656311819397,"score_gpt":0.2142682229217387,"score_spread":0.2089216598035447,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}