{"id":"W2064578970","doi":"10.1002/pro.512","title":"Secretion of human superoxide dismutase in <i>Escherichia coli</i> using the condensed single‐protein‐production system","year":2010,"lang":"en","type":"article","venue":"Protein Science","topic":"Lipid Membrane Structure and Behavior","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto; Ontario Institute for Cancer Research","funders":"Takara Bio; National Institute of General Medical Sciences","keywords":"Periplasmic space; DsbA; Escherichia coli; Superoxide dismutase; Signal peptide; Heteronuclear single quantum coherence spectroscopy; Biochemistry; Chemistry; Heteronuclear molecule; Peptide; Biology; Enzyme; Recombinant DNA; Nuclear magnetic resonance spectroscopy; Stereochemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007261583,0.0001194657,0.0001190056,0.00005775561,0.0002062944,0.00003853038,0.0003459147,0.00009250297,0.00000570532],"category_scores_gemma":[0.0002034015,0.00008778933,0.00004115274,0.0003736712,0.000665491,0.00001940638,0.00009219549,0.0001677132,7.952618e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003138056,"about_ca_system_score_gemma":0.0001724663,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002519782,"about_ca_topic_score_gemma":0.0001645615,"domain_scores_codex":[0.9987883,0.00005970351,0.0002370442,0.0003996815,0.000278751,0.000236552],"domain_scores_gemma":[0.9991618,0.000002596825,0.0001252677,0.0004723632,0.00018325,0.00005470213],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001531347,0.00003070389,0.0002220105,0.00002804264,0.000001561496,9.822005e-7,0.00006791519,0.00000968214,0.9990768,0.0004562707,0.000001886272,0.00008880466],"study_design_scores_gemma":[0.000186623,0.00009555207,0.0004248615,0.0000370489,0.000008389245,0.00001377,0.0002450326,0.00006660605,0.9986823,0.00002274141,0.0001025591,0.0001145227],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9986374,0.00001978082,0.0001112874,0.00008917677,0.0002938528,0.0007027054,0.000005491305,0.00001062728,0.0001296898],"genre_scores_gemma":[0.9989108,3.120915e-7,0.0007069494,0.00002941849,0.0002365117,0.00003965863,0.000007084637,0.00001026448,0.00005899989],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006630467,"threshold_uncertainty_score":0.3579945,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01545156882820406,"score_gpt":0.2617137678660363,"score_spread":0.2462621990378322,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}