{"id":"W2064612471","doi":"10.1251/bpo119","title":"Practical three color live cell imaging by widefield microscopy","year":2006,"lang":"en","type":"article","venue":"Biological Procedures Online","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"McMaster University","funders":"Canadian Institutes of Health Research","keywords":"Fluorophore; Aequorea victoria; Fluorescence; Fluorescence microscope; Green fluorescent protein; Microscope; Microscopy; Live cell imaging; Cytoplasm; Biophysics; Fusion protein; Cell biology; Protein subcellular localization prediction; Chemistry; Biology; Cell; Optics; Biochemistry; Physics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005070505,0.0006831767,0.0003762068,0.000442597,0.0002996358,0.0005594437,0.0008273172,0.0007735884,0.003453727],"category_scores_gemma":[0.0004405949,0.0004166891,0.0002744418,0.0003037717,0.0004899956,0.0008919346,0.0009902942,0.001290041,0.001668462],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003623978,"about_ca_system_score_gemma":0.0002412937,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004083215,"about_ca_topic_score_gemma":0.0005362769,"domain_scores_codex":[0.9996014,0.00005571987,0.00001986977,0.0001108472,0.0001736512,0.00003853424],"domain_scores_gemma":[0.9996171,0.0001376366,0.00003752086,0.00008491765,0.0000839529,0.000038821],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002499686,0.000009906879,0.00008058712,0.00004394405,0.000002661251,0.00003237612,0.00001642605,0.0002612215,0.9932988,0.00062822,0.0001986945,0.005402292],"study_design_scores_gemma":[0.00001488475,0.00006612617,0.001014556,0.000009364226,0.000006306071,0.0007691864,0.00003131068,0.01088917,0.9796444,0.001057131,0.0064733,0.00002431152],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1462803,0.001273701,0.8423251,0.0003738263,0.00008077425,0.0001302714,0.000495117,0.001800761,0.007240084],"genre_scores_gemma":[0.3488071,0.002352646,0.6388739,0.0002447951,0.00005218585,0.0004564358,0.0009402689,0.0002705496,0.008002146],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003453727,"threshold_uncertainty_score":0.01155388,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01195626103252902,"score_gpt":0.3104636833274865,"score_spread":0.2985074222949575,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}