{"id":"W2064765153","doi":"10.1371/journal.pone.0044908","title":"Highly Efficient Isolation of Populus Mesophyll Protoplasts and Its Application in Transient Expression Assays","year":2012,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Plant Molecular Biology Research","field":"Agricultural and Biological Sciences","cited_by":108,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Oak Ridge National Laboratory; Biological and Environmental Research; Natural Sciences and Engineering Research Council of Canada; Office of Science; Purdue University; UT-Battelle; Battelle; Arizona Biomedical Research Commission; U.S. Department of Energy","keywords":"Protoplast; Biology; Botany; Cell biology; Gene expression; Gene; Biochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002504643,0.00005883234,0.0001089137,0.0000178901,0.00003527591,0.000004110541,0.00007124717,0.00007736255,0.00001080374],"category_scores_gemma":[0.000033371,0.00002474295,0.00001411751,0.0001486334,0.00001808986,0.00004161665,0.00003353841,0.00007293726,0.000008401694],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001319641,"about_ca_system_score_gemma":0.0000024114,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002055887,"about_ca_topic_score_gemma":0.0000219196,"domain_scores_codex":[0.9992717,0.00009213294,0.0001305837,0.000138588,0.0001889438,0.000178058],"domain_scores_gemma":[0.9997717,0.00006143848,0.00004715782,0.00002999476,0.0000312608,0.0000584206],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004098533,0.0006258907,0.03798235,0.00001852287,0.000003823883,2.405773e-7,0.00008311517,0.000002688796,0.9602892,0.0000493757,0.000001907494,0.0009019353],"study_design_scores_gemma":[0.0001201335,0.0001214515,0.3317781,0.00004777748,0.000005221239,9.929755e-7,0.00001718816,0.00137089,0.6664407,0.00001611741,0.00002167503,0.00005974037],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.998594,0.000251463,0.00001571586,0.0002282217,0.00000653343,0.0007516685,0.00001810362,0.00001223539,0.0001219884],"genre_scores_gemma":[0.9996408,0.0000175414,0.0001274468,0.0000116752,0.00003291764,0.0001096851,0.00004631665,5.279371e-7,0.00001314662],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2938484,"threshold_uncertainty_score":0.1008988,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03417810228216245,"score_gpt":0.2325061703691587,"score_spread":0.1983280680869963,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}