{"id":"W2064969434","doi":"10.1073/pnas.0405054101","title":"Two perfectly conserved arginine residues are required for substrate binding in a high-affinity nitrate transporter","year":2004,"lang":"en","type":"article","venue":"Proceedings of the National Academy of Sciences","topic":"Plant nutrient uptake and metabolism","field":"Agricultural and Biological Sciences","cited_by":48,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; University of British Columbia; TRIUMF","keywords":"Major facilitator superfamily; Transmembrane protein; Transmembrane domain; Nitrate; Biochemistry; Salt bridge; Transporter; Lysine; Amino acid; Chemistry; Binding site; Arginine; Mutant; Biology; Gene; Receptor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001285365,0.0001113108,0.000223404,0.00005928772,0.0002081691,0.00002995497,0.0005419673,0.00007952651,0.000009444558],"category_scores_gemma":[0.0001877028,0.00004291126,0.00009492439,0.0008434413,0.0003590407,0.0004212304,0.00002651313,0.0001220884,8.184048e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001859436,"about_ca_system_score_gemma":0.00001972328,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006764563,"about_ca_topic_score_gemma":0.00002439331,"domain_scores_codex":[0.9985977,0.00000794329,0.0003677362,0.0002651816,0.0005310286,0.0002304197],"domain_scores_gemma":[0.9992311,0.0001576831,0.0004005687,0.000003657854,0.0001662324,0.00004076959],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"observational","study_design_scores_codex":[0.00007604504,0.00007308934,0.0340441,0.00001797349,0.000006128194,3.231103e-8,0.0000855155,0.00003160643,0.943011,0.02243677,0.00005190551,0.0001658486],"study_design_scores_gemma":[0.00051805,0.00006376641,0.4989582,0.0001286197,0.000009501269,0.000003110222,0.0002514775,0.0000116918,0.4605253,0.03929094,0.000151273,0.00008808197],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9943339,0.0001328324,7.483718e-8,0.004597966,0.00002522818,0.0002897902,0.0001620739,0.00001435425,0.0004437484],"genre_scores_gemma":[0.9991409,0.0001009317,0.0003732636,0.0002091913,0.00009944942,0.00002026504,0.000002355161,6.727819e-7,0.00005303831],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.4824857,"threshold_uncertainty_score":0.174987,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.06485859091789138,"score_gpt":0.2745833740383228,"score_spread":0.2097247831204315,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}