{"id":"W2066061472","doi":"10.1107/s1744309111043910","title":"Expression, purification, crystallization and preliminary crystallographic analysis of PilA from the nontypeable<i>Haemophilus influenzae</i>type IV pilus","year":2012,"lang":"en","type":"article","venue":"Acta Crystallographica Section F Structural Biology and Crystallization Communications","topic":"Peptidase Inhibition and Analysis","field":"Medicine","cited_by":4,"is_retracted":false,"has_abstract":true,"ca_institutions":"Simon Fraser University","funders":"National Institute on Deafness and Other Communication Disorders; Canadian Institutes of Health Research; National Institutes of Health; Michael Smith Health Research BC; Canadian Light Source","keywords":"Pilin; Pilus; Crystallization; Haemophilus influenzae; Microbiology; Crystallography; Protein subunit; Biology; Biofilm; Fimbriae Proteins; Chemistry; Virulence; Bacteria; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004622855,0.0003357225,0.000411199,0.0003296577,0.0004513788,0.0004172841,0.000448074,0.0002087863,0.0006334689],"category_scores_gemma":[0.0007874396,0.0001866512,0.0003329317,0.0009784997,0.0002608583,0.0002289311,0.0002398316,0.0007699871,0.0007196153],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006741134,"about_ca_system_score_gemma":0.0006417399,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.005097559,"about_ca_topic_score_gemma":0.004361877,"domain_scores_codex":[0.9996667,0.0000625943,0.00004475499,0.00003814744,0.0001202139,0.00006748271],"domain_scores_gemma":[0.9997846,0.0000448279,0.00002481067,0.00003905043,0.00007150201,0.00003523753],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004628051,0.0001681289,0.001593324,0.0001975562,0.00001396358,0.0004056561,0.0002068626,0.001374778,0.9872288,0.0006380656,0.0006715305,0.007038509],"study_design_scores_gemma":[0.0000384854,0.0002479122,0.01535913,0.0000174105,0.00003379124,0.0007292225,0.0001752352,0.00344198,0.9608383,0.0003157481,0.01878614,0.0000167112],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9724689,0.002878324,0.01243617,0.0006251401,0.00006671242,0.0003686104,0.007830814,0.0001246832,0.003200762],"genre_scores_gemma":[0.8958215,0.005259243,0.05635382,0.0001463564,0.00004735111,0.0002748797,0.0358394,0.0001594977,0.006098176],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005097559,"threshold_uncertainty_score":0.01013577,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0217004996296235,"score_gpt":0.2844364702967456,"score_spread":0.2627359706671221,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}