{"id":"W2066672437","doi":"10.1085/jgp.200910347","title":"Rapid topology probing using fluorescence spectroscopy in planar lipid bilayer: the pore-forming mechanism of the toxin Cry1Aa of <i>Bacillus thuringiensis</i>","year":2010,"lang":"en","type":"article","venue":"The Journal of General Physiology","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"Fonds Québécois de la Recherche sur la Nature et les Technologies; Canada Research Chairs","keywords":"Förster resonance energy transfer; Lipid bilayer; Bacillus thuringiensis; Biophysics; Membrane; Chemistry; Bilayer; Single-molecule FRET; Membrane topology; Fluorescence; Vesicle; Membrane protein; Crystallography; Topology (electrical circuits); Biology; Biochemistry; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001394603,0.0002988424,0.0001551132,0.0001672946,0.0002072105,0.0002550127,0.0002685993,0.0004106361,0.0004634232],"category_scores_gemma":[0.0001957941,0.0001337614,0.000166358,0.0001663833,0.0003197903,0.0004460081,0.000295939,0.0005065789,0.0001485102],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002906393,"about_ca_system_score_gemma":0.0001277785,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005093832,"about_ca_topic_score_gemma":0.0003684024,"domain_scores_codex":[0.9999046,0.00001211267,0.000003658102,0.00003280204,0.00002692259,0.00001980231],"domain_scores_gemma":[0.9999167,0.00002526979,0.00002693221,0.000005613772,0.00001248139,0.00001303194],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001578618,0.000005113877,0.0001444376,0.00001989205,0.000001676032,0.00001922754,0.00002143582,0.00007789894,0.9990746,0.00005635148,0.0000161499,0.000547398],"study_design_scores_gemma":[0.00001366875,0.0001358538,0.004036717,0.000006065547,0.000007451252,0.000213562,0.00008205798,0.004017688,0.9907811,0.0001112826,0.0005821227,0.00001236612],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9906375,0.0004128718,0.008003337,0.00009243834,0.00001091578,0.00002049095,0.0001579672,0.00006967684,0.0005949151],"genre_scores_gemma":[0.9896792,0.0005859917,0.009028111,0.00004980141,0.000007742766,0.0000333196,0.0001619339,0.00001950177,0.0004342683],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005093832,"threshold_uncertainty_score":0.002108693,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008976581675663578,"score_gpt":0.233667708869196,"score_spread":0.2246911271935325,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}