{"id":"W2067161271","doi":"10.1634/stemcells.2007-0801","title":"Alkaline Phosphatase-Positive Colony Formation Is a Sensitive, Specific, and Quantitative Indicator of Undifferentiated Human Embryonic Stem Cells","year":2008,"lang":"en","type":"article","venue":"Stem Cells","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":168,"is_retracted":false,"has_abstract":true,"ca_institutions":"Lunenfeld-Tanenbaum Research Institute; Mount Sinai Hospital; BC Cancer Agency","funders":"Canadian Institutes of Health Research; University of British Columbia; Michael Smith Health Research BC; Stem Cell Network; Faculty of Medicine, University of British Columbia","keywords":"Biology; Embryoid body; Clonogenic assay; Homeobox protein NANOG; Embryonic stem cell; Alkaline phosphatase; Induced pluripotent stem cell; Cell biology; KOSR; Stem cell; SOX2; Cellular differentiation; Retinoic acid; Population; Molecular biology; In vitro; Cell culture; Biochemistry; Genetics; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006745697,0.0009968508,0.0005274017,0.001582677,0.0003147817,0.0005938126,0.0007508601,0.0004193034,0.001536193],"category_scores_gemma":[0.0006757037,0.0004850781,0.0003906823,0.0008274619,0.0006438592,0.0003792063,0.001066039,0.00106625,0.001822705],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002939325,"about_ca_system_score_gemma":0.0003743892,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000512571,"about_ca_topic_score_gemma":0.001150667,"domain_scores_codex":[0.9989333,0.0002155867,0.000101269,0.0001521492,0.0005332159,0.00006452463],"domain_scores_gemma":[0.9987805,0.0003107765,0.0002351302,0.0001607722,0.0003560448,0.0001567568],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005622011,0.00005047195,0.001341486,0.0001674362,0.00001137155,0.00009900303,0.00009694917,0.00008075801,0.9862498,0.0005146212,0.0004428646,0.01088896],"study_design_scores_gemma":[0.000009296859,0.000124901,0.004702146,0.00002245959,0.00001822745,0.0005949829,0.00004450907,0.001368431,0.9852298,0.0003049162,0.007569212,0.00001118931],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4522671,0.01641488,0.4949461,0.0006378855,0.0004885449,0.0006303523,0.002850815,0.005206178,0.02655823],"genre_scores_gemma":[0.6626264,0.01090575,0.2959643,0.0002846268,0.0001428453,0.0009965635,0.004588821,0.0005168407,0.02397391],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.001582677,"threshold_uncertainty_score":0.005139053,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02496271950428851,"score_gpt":0.2652766108826863,"score_spread":0.2403138913783978,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}