{"id":"W2068391719","doi":"10.1007/s10295-004-0181-2","title":"The effect of nitrogen source on yield and glycosylation of a human cystatin C mutant expressed in Pichia pastoris","year":2004,"lang":"en","type":"article","venue":"Journal of Industrial Microbiology & Biotechnology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":20,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Pichia pastoris; Glycosylation; Biochemistry; Chemistry; Cystatin; Mutant; Amino acid; Ammonium hydroxide; Cystatin C; Recombinant DNA; Organic chemistry; Gene; Creatinine","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004460419,0.0001133244,0.0002693074,0.0001640211,0.00005035294,0.000004066032,0.0001611269,0.0006008719,0.000002853294],"category_scores_gemma":[0.0004689241,0.00007940706,0.0000631736,0.0001287403,0.0002445776,0.000004167049,0.00005157453,0.0003374194,3.408099e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002358635,"about_ca_system_score_gemma":0.00004903309,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002387496,"about_ca_topic_score_gemma":0.00002656431,"domain_scores_codex":[0.9990358,0.0001256109,0.000518193,0.0001504151,0.00003837718,0.0001315861],"domain_scores_gemma":[0.9990464,0.00005098192,0.0006507856,0.0001655226,0.00006637289,0.00001989838],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0008743131,0.00004117705,0.003982571,0.000005994002,0.00004998786,0.000001523764,0.00003283081,0.00005081769,0.9911246,0.00006595963,0.0000577415,0.003712494],"study_design_scores_gemma":[0.002072193,0.004311495,0.0001977409,0.00005960549,0.00001997057,0.00008864383,0.00005623046,4.066002e-7,0.9907387,0.00008724004,0.002306294,0.00006146451],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9979883,0.0002455596,0.0002070426,0.001165682,0.0002161157,0.0001557949,0.0000134409,0.00000353251,0.000004527559],"genre_scores_gemma":[0.9995915,0.0001663956,0.00004372652,0.00002194946,0.0001440889,0.000002590234,0.0000153886,0.000008310346,0.000006030286],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004270317,"threshold_uncertainty_score":0.4634474,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008380150152736956,"score_gpt":0.2234544198026764,"score_spread":0.2150742696499394,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}