{"id":"W2069581732","doi":"10.1016/j.bpj.2008.12.3390","title":"Single-molecule Force Spectroscopy Reveals Engineered Metal Chelation Is A General Approach To Enhance Mechanical Stability Of Proteins","year":2009,"lang":"en","type":"article","venue":"Biophysical Journal","topic":"Force Microscopy Techniques and Applications","field":"Physics and Astronomy","cited_by":1,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"","keywords":"Force spectroscopy; Chelation; Protein engineering; Chemistry; Histidine; Nanotechnology; Materials science; Metal ions in aqueous solution; Native state; Metal; Combinatorial chemistry; Crystallography; Atomic force microscopy; Biochemistry; Organic chemistry; Amino acid","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003233079,0.0003221299,0.0003128045,0.0001619789,0.0002806791,0.0004225105,0.0004268765,0.0006872785,0.0008331386],"category_scores_gemma":[0.000339025,0.0002632838,0.000190666,0.0001074323,0.0004036625,0.0004269958,0.000222434,0.0006735012,0.0002368201],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003646575,"about_ca_system_score_gemma":0.0001602467,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003392216,"about_ca_topic_score_gemma":0.0008722075,"domain_scores_codex":[0.9998304,0.00001575718,0.000010868,0.00006075205,0.00005844669,0.00002382084],"domain_scores_gemma":[0.9998386,0.00004606936,0.00004798091,0.00002048318,0.00002056627,0.00002622158],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002020712,0.000009838156,0.00005638132,0.00002383198,0.000003060187,0.00001418488,0.00001037184,0.00007106044,0.9986194,0.0001455713,0.00007241003,0.000953546],"study_design_scores_gemma":[0.00000677052,0.00003088246,0.0002769135,0.000001502784,0.000002414493,0.00004194043,0.000003130103,0.001020591,0.9981418,0.00005911368,0.0004119978,0.000002949188],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9705602,0.0009768852,0.02567334,0.0006829273,0.00007803727,0.00003531903,0.0002151791,0.0003659238,0.001412256],"genre_scores_gemma":[0.9878752,0.0004469909,0.009667594,0.000255322,0.0000216531,0.00003464303,0.0001236301,0.00003796977,0.001537061],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008331386,"threshold_uncertainty_score":0.002787054,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01282137667282585,"score_gpt":0.2809057141894718,"score_spread":0.268084337516646,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}