{"id":"W2069698969","doi":"10.3791/2387","title":"Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection","year":2010,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Canadian Institutes of Health Research","keywords":"Biology; Messenger RNA; Molecular biology; Microinjection; RNA; Cell biology; Transfection; Xenopus; Cell nucleus; Transcription (linguistics); Precursor mRNA; Gene expression; RNA splicing; Cytoplasm; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005059989,0.0004662612,0.0006935737,0.0007783433,0.0003050222,0.0006454546,0.000561763,0.0004901497,0.001611016],"category_scores_gemma":[0.0004406008,0.0003660826,0.0006405532,0.0006862513,0.0003764571,0.000437119,0.0003552057,0.001362177,0.001500571],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006726133,"about_ca_system_score_gemma":0.0003043071,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001381569,"about_ca_topic_score_gemma":0.001552241,"domain_scores_codex":[0.999589,0.00004361778,0.0000490945,0.0001511137,0.0001217589,0.00004533818],"domain_scores_gemma":[0.9995033,0.000217533,0.0000830519,0.00006743751,0.00008836968,0.00004031081],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002933935,0.00001266423,0.000117178,0.0000295168,0.000003887019,0.00001918956,0.00003493414,0.00009679241,0.9984856,0.0001480509,0.0000258987,0.000996894],"study_design_scores_gemma":[0.00000921921,0.000161789,0.002535752,0.000007027157,0.00001755375,0.00010487,0.00003310723,0.002270327,0.9920325,0.00009095833,0.002724101,0.00001282025],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7526012,0.008245043,0.2205258,0.0002618328,0.0002329597,0.0006184304,0.005180645,0.001971507,0.0103625],"genre_scores_gemma":[0.8087628,0.009082953,0.157177,0.0002009725,0.00008679927,0.00124881,0.006990308,0.0009878846,0.01546237],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001611016,"threshold_uncertainty_score":0.005389392,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01203437189832277,"score_gpt":0.3737353066638048,"score_spread":0.361700934765482,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}