{"id":"W2069844653","doi":"10.1021/ac025742k","title":"Recombinant <i>Gaussia</i> Luciferase. Overexpression, Purification, and Analytical Application of a Bioluminescent Reporter for DNA Hybridization","year":2002,"lang":"en","type":"article","venue":"Analytical Chemistry","topic":"bioluminescence and chemiluminescence research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":214,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Windsor","funders":"","keywords":"Chemistry; Complementary DNA; Luciferase; Biotinylation; Molecular biology; Biotin; Biochemistry; Bioluminescence; Biology; Transfection; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004779395,0.0006373373,0.0003600753,0.0003158513,0.0002142709,0.0004618213,0.0005020002,0.0004536745,0.0006743611],"category_scores_gemma":[0.0004001672,0.0003454493,0.0004894746,0.0003861125,0.0003846133,0.0002963883,0.0004593532,0.0008879658,0.001352371],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006815994,"about_ca_system_score_gemma":0.0003845328,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008541328,"about_ca_topic_score_gemma":0.0006156482,"domain_scores_codex":[0.9995846,0.00008880463,0.00004213469,0.00009816146,0.0001192321,0.00006691401],"domain_scores_gemma":[0.9997967,0.00004335839,0.00005461791,0.00003512964,0.0000408571,0.00002934296],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001800818,0.00000894051,0.00006987106,0.00005816722,0.000002405673,0.00001604514,0.000008480445,0.00004212465,0.9986793,0.0000997906,0.0000717112,0.0009252327],"study_design_scores_gemma":[0.000006312413,0.00005307999,0.0007907594,0.000007403165,0.000008076487,0.0001669176,0.000007504574,0.0006437556,0.9920036,0.00005623446,0.006250059,0.000006340148],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6572323,0.007221351,0.31156,0.001028387,0.0002807578,0.0007655505,0.00875135,0.003305077,0.009855188],"genre_scores_gemma":[0.7247666,0.007254905,0.2188772,0.0004753726,0.00009685607,0.0009153325,0.03238684,0.0006996393,0.01452725],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008541328,"threshold_uncertainty_score":0.004945338,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01701188270058917,"score_gpt":0.2708653835774094,"score_spread":0.2538535008768202,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}