{"id":"W2069844653","doi":"10.1021/ac025742k","title":"Recombinant <i>Gaussia</i> Luciferase. Overexpression, Purification, and Analytical Application of a Bioluminescent Reporter for DNA Hybridization","year":2002,"lang":"en","type":"article","venue":"Analytical Chemistry","topic":"bioluminescence and chemiluminescence research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":214,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Windsor","funders":"","keywords":"Chemistry; Complementary DNA; Luciferase; Biotinylation; Molecular biology; Biotin; Biochemistry; Bioluminescence; Biology; Transfection; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002177081,0.0001991697,0.0002537694,0.00003134349,0.0000853158,0.00002727179,0.0002005548,0.0002556296,0.00005457618],"category_scores_gemma":[0.000389996,0.0001803488,0.0001262975,0.0002065797,0.0002666646,0.00001355125,0.00008568165,0.0000915648,0.000003282936],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002167346,"about_ca_system_score_gemma":0.00003804181,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000006249338,"about_ca_topic_score_gemma":8.497595e-7,"domain_scores_codex":[0.9982976,0.00001740966,0.0005118846,0.0006179047,0.0002345347,0.0003207063],"domain_scores_gemma":[0.9987022,0.00003179093,0.0001766583,0.0005552087,0.0003351591,0.0001990226],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007307793,0.0002912538,0.01200456,0.0002626774,0.00004467977,0.000001251792,0.000009845198,0.0000010365,0.9714475,0.000109004,0.01404205,0.001713118],"study_design_scores_gemma":[0.0006215777,0.00009737801,0.0008879742,0.00006377064,0.00006067728,0.00003545711,0.00004728304,0.01597164,0.9616644,0.00008088359,0.02018496,0.0002840338],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9858711,0.0006134334,0.009221458,0.001496015,0.00003866204,0.0005745911,0.00005095917,0.00002871337,0.002105023],"genre_scores_gemma":[0.9961842,0.0003232346,0.0002789862,0.0000860786,0.0002139184,0.00008569978,0.000407798,0.00002330507,0.002396725],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0159706,"threshold_uncertainty_score":0.735441,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01701188270058917,"score_gpt":0.2708653835774094,"score_spread":0.2538535008768202,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}