{"id":"W2070860432","doi":"10.1186/1472-6750-13-7","title":"Development of a versatile TaqMan™ real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA","year":2013,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Moncton","funders":"Institute of Genetics; Natural Sciences and Engineering Research Council of Canada","keywords":"Biology; genomic DNA; TaqMan; RNA; Gene; DNA; Complementary DNA; GenBank; Molecular biology; Computational biology; Genetics; Real-time polymerase chain reaction","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002621265,0.0012803,0.001108443,0.001063377,0.00030233,0.0008540452,0.001117987,0.001436833,0.001636506],"category_scores_gemma":[0.002733789,0.0008153551,0.00135846,0.0006041371,0.00081422,0.000726901,0.0008319931,0.001708517,0.002172791],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005467145,"about_ca_system_score_gemma":0.0008885831,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001944626,"about_ca_topic_score_gemma":0.0003752632,"domain_scores_codex":[0.9973589,0.0003673806,0.0002150112,0.0009428805,0.0009130705,0.0002028037],"domain_scores_gemma":[0.9982898,0.0005356975,0.0004188443,0.0002295649,0.0004022183,0.0001238392],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003374738,0.00001886007,0.0001387914,0.0001020166,0.000007374813,0.00003290751,0.00002074556,0.0002448695,0.9960084,0.0001928779,0.00009171791,0.003107759],"study_design_scores_gemma":[0.00001464101,0.0003180187,0.0004611767,0.00002329839,0.00003035333,0.0001954274,0.00001603044,0.002770016,0.9904261,0.0001339174,0.005586488,0.00002448653],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.1208608,0.0009260196,0.8706517,0.0003367459,0.0003735439,0.0009316388,0.001649446,0.002925212,0.001344952],"genre_scores_gemma":[0.181094,0.001038349,0.8046765,0.0003294271,0.0000927668,0.002251949,0.00531623,0.0004785759,0.004722281],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002621265,"threshold_uncertainty_score":0.01386273,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05547854225590671,"score_gpt":0.2730206652593249,"score_spread":0.2175421230034182,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}