{"id":"W2070860432","doi":"10.1186/1472-6750-13-7","title":"Development of a versatile TaqMan™ real-time quantitative PCR (RT-qPCR) compliant anchor sequence to quantify bacterial gene transcripts from RNA samples containing carryover genomic DNA","year":2013,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Moncton","funders":"Institute of Genetics; Natural Sciences and Engineering Research Council of Canada","keywords":"Biology; genomic DNA; TaqMan; RNA; Gene; DNA; Complementary DNA; GenBank; Molecular biology; Computational biology; Genetics; Real-time polymerase chain reaction","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0001509332,0.0002552774,0.0003632226,0.0001093837,0.000109682,0.00002162622,0.0003285605,0.0004562585,0.000106746],"category_scores_gemma":[0.00007248301,0.0002467193,0.00008717087,0.0001299059,0.0002227174,0.000007086154,0.0001741899,0.0001148366,0.00009843684],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005491844,"about_ca_system_score_gemma":0.0002157584,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000703693,"about_ca_topic_score_gemma":0.0003189927,"domain_scores_codex":[0.9982125,0.00005784002,0.0004788834,0.0007371469,0.0001209798,0.0003926185],"domain_scores_gemma":[0.9990597,0.00001760778,0.0001970379,0.0004970921,0.0001341043,0.00009447878],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003005368,0.00005249883,0.0003580019,0.00001655566,0.0001022221,0.000001263332,0.0004264663,0.00003176569,0.9964082,0.0002099077,0.0001810044,0.001911632],"study_design_scores_gemma":[0.000571275,0.0006359648,0.02167617,0.00002397889,0.00002407369,0.000006820995,0.0005418432,0.0001119284,0.9671973,0.00006075874,0.008843039,0.0003068617],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9839915,0.0002436883,0.01447964,0.0002413889,0.0002701961,0.0005269975,0.0001502944,0.00004303172,0.00005323198],"genre_scores_gemma":[0.8226468,0.0001215141,0.1766744,0.00004497423,0.0001146104,0.00004886201,0.0002558166,0.0000311414,0.00006191165],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1621947,"threshold_uncertainty_score":0.9999985,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05547854225590671,"score_gpt":0.2730206652593249,"score_spread":0.2175421230034182,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}