{"id":"W2071151402","doi":"10.1210/mend.15.11.0730","title":"Critical Residues for the Specificity of Cofactors and Substrates in Human Estrogenic 17β-Hydroxysteroid Dehydrogenase 1: Variants Designed from the Three-Dimensional Structure of the Enzyme","year":2001,"lang":"en","type":"article","venue":"Molecular Endocrinology","topic":"Estrogen and related hormone effects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":63,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval; Medical Council of Canada","funders":"","keywords":"Cofactor; NAD+ kinase; Biochemistry; Enzyme; Biology; Dehydrogenase; Oxidoreductase; Reductase; Lysine; Amino acid; Cysteine; Stereochemistry; Chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001769888,0.0006390812,0.0004686071,0.0001351084,0.000150706,0.0003917905,0.0004234587,0.0003897874,0.0008926186],"category_scores_gemma":[0.000325573,0.0002827384,0.0004153031,0.0002245628,0.0003361995,0.0001968912,0.0002808414,0.0004176485,0.0004873932],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000346011,"about_ca_system_score_gemma":0.000272415,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006079134,"about_ca_topic_score_gemma":0.0008520068,"domain_scores_codex":[0.9997925,0.0000544711,0.00003252301,0.00004590471,0.00004831384,0.00002626771],"domain_scores_gemma":[0.9997733,0.00004700908,0.00007669943,0.0000227935,0.00002772555,0.00005233795],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007002708,0.00007574893,0.001194565,0.00005797424,0.00002335029,0.0002446813,0.00004005668,0.0005417271,0.9949797,0.0002093634,0.00004346209,0.001889107],"study_design_scores_gemma":[0.0001035247,0.0005174983,0.008098672,0.00001101139,0.00007612894,0.001593792,0.00004554936,0.002478744,0.9843836,0.0001235574,0.002538941,0.00002893646],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9952689,0.0006589997,0.00317643,0.00005752707,0.00003199812,0.0000486201,0.0002357739,0.00004536086,0.0004763785],"genre_scores_gemma":[0.994135,0.0002426453,0.004322434,0.00004741619,0.000009391042,0.00002454543,0.0005348196,0.00003825099,0.0006455918],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008926186,"threshold_uncertainty_score":0.002986073,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01031026580177557,"score_gpt":0.2490713694401353,"score_spread":0.2387611036383597,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}