{"id":"W2071726080","doi":"10.1016/j.yexmp.2011.09.013","title":"Nucleic acid quantity and quality from paraffin blocks: Defining optimal fixation, processing and DNA/RNA extraction techniques","year":2011,"lang":"en","type":"article","venue":"Experimental and Molecular Pathology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":116,"is_retracted":false,"has_abstract":false,"ca_institutions":"Vancouver General Hospital; University of British Columbia; Provincial Health Services Authority; BC Cancer Agency","funders":"Canadian Institutes of Health Research; Canada Research Chairs","keywords":"Fixative; Amplicon; Fixation (population genetics); Nucleic acid; Biology; RNA; RNA extraction; DNA; DNA extraction; Polymerase chain reaction; Biochemistry; Gene; Genetics; Staining","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0153374,0.0009362021,0.001528143,0.002172213,0.0009561864,0.00272335,0.001744544,0.001361525,0.001022433],"category_scores_gemma":[0.01881883,0.001275342,0.0004363993,0.001420853,0.00178575,0.003273642,0.001210505,0.001095998,0.0006603745],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001176828,"about_ca_system_score_gemma":0.002308556,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001244531,"about_ca_topic_score_gemma":0.00277681,"domain_scores_codex":[0.9917938,0.002315247,0.001852067,0.001055485,0.002632825,0.0003505489],"domain_scores_gemma":[0.9824808,0.008255354,0.002644649,0.001315379,0.004860396,0.0004432792],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006255187,0.0001544803,0.008126038,0.0008661064,0.00003253672,0.0001211801,0.0002502596,0.001579134,0.9261975,0.002547348,0.0004803305,0.05901948],"study_design_scores_gemma":[0.00004662723,0.0008383418,0.01852658,0.0003676698,0.0002611972,0.001069768,0.0003049686,0.01088215,0.954904,0.002770376,0.009944644,0.0000835759],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.24775,0.02369263,0.7183833,0.001444241,0.0002549849,0.001922696,0.000745004,0.00126745,0.004539726],"genre_scores_gemma":[0.2452318,0.009592039,0.7398545,0.0003368318,0.0001868788,0.001105421,0.001064134,0.0007210596,0.001907503],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.0153374,"threshold_uncertainty_score":0.08111286,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02046634995989694,"score_gpt":0.308103492974978,"score_spread":0.287637143015081,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}