{"id":"W2071752319","doi":"10.1006/bbrc.2001.4717","title":"Cloning and Expression of Human Rotavirus Spike Protein, VP8*, in Escherichia coli","year":2001,"lang":"en","type":"article","venue":"Biochemical and Biophysical Research Communications","topic":"Viral gastroenteritis research and epidemiology","field":"Medicine","cited_by":26,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Escherichia coli; Fusion protein; Molecular biology; Complementary DNA; Plasmid; Biology; Affinity chromatography; Cloning (programming); Expression vector; Sepharose; Recombinant DNA; Virology; Chemistry; Biochemistry; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002807108,0.0003877553,0.0005086629,0.0003160495,0.000290929,0.0006446523,0.0004373013,0.0002910039,0.0006285384],"category_scores_gemma":[0.0003428133,0.0003075973,0.0004448305,0.0005455057,0.0001884699,0.000261737,0.0003220098,0.0007330973,0.0008769831],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006525058,"about_ca_system_score_gemma":0.0003674678,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002465264,"about_ca_topic_score_gemma":0.001578211,"domain_scores_codex":[0.9997774,0.00004385228,0.00002935409,0.00003617281,0.00006142232,0.00005185262],"domain_scores_gemma":[0.9998324,0.00004843373,0.00002502225,0.00003472737,0.00003466873,0.00002466625],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002748192,0.000084531,0.000400581,0.00006857722,0.000009903531,0.0001122431,0.00008556838,0.0002689776,0.9958877,0.0003479261,0.0001432001,0.002315987],"study_design_scores_gemma":[0.00003724944,0.0002975104,0.002745328,0.00002067058,0.0000225258,0.0002960477,0.000125787,0.001979415,0.9884896,0.0001334527,0.005840247,0.00001225155],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9891871,0.000665531,0.006635614,0.0001675154,0.00007426281,0.00006976831,0.001623055,0.0001165074,0.001460622],"genre_scores_gemma":[0.9498035,0.001324154,0.02445217,0.0001054704,0.00003995323,0.0001041648,0.01331846,0.0001383886,0.01071376],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002465264,"threshold_uncertainty_score":0.004901826,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.1193459991783376,"score_gpt":0.4295853046907722,"score_spread":0.3102393055124346,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}