{"id":"W2072736827","doi":"10.1016/s0006-2952(01)00742-0","title":"Sequestration and phosphorylation of the prostaglandin E2 EP4 receptor: dependence on the C-terminal tail33Abbreviations: PGE2, prostaglandin E2, GRK, G-protein coupled receptor kinase; PKA, protein kinase A; PKC, protein kinase C; PCR, polymerase chain reaction; DMEM, Dulbecco’s modified Eagle’s medium; PAS, protein A Sepharose; ECL, electrochemiluminescence; BCA, 2-bicinchoninic acid; IR, immunoreactivity; HBSS, Hanks’ buffered salt solution; TBS, Tris-buffered saline; cAMP, cyclic AMP; PMA, phorbol 12-myristate 13 acetate; and β2-AR, β2-adrenergic receptor.","year":2001,"lang":"en","type":"article","venue":"Biochemical Pharmacology","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":false,"ca_institutions":"TransCanada (Canada)","funders":"","keywords":"Prostaglandin E2 receptor; Forskolin; Protein kinase C; Protein kinase A; Homologous desensitization; Phosphorylation; Adenylyl cyclase; Cell biology; Receptor; Chemistry; Biology; Prostaglandin E2; Agonist; Molecular biology; Signal transduction; Biochemistry; Endocrinology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001512183,0.0003009714,0.0001480775,0.000147852,0.0001949135,0.0002245371,0.0002726405,0.0002498754,0.005891538],"category_scores_gemma":[0.0002095402,0.0001945338,0.000282712,0.0001447318,0.0002021483,0.000307731,0.0002967528,0.0004301534,0.001787919],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000505537,"about_ca_system_score_gemma":0.0002544774,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007975291,"about_ca_topic_score_gemma":0.00101169,"domain_scores_codex":[0.9998635,0.00001750969,0.00001016327,0.00002327772,0.00002186001,0.00006369285],"domain_scores_gemma":[0.9999167,0.00001609435,0.00002045605,0.00001495542,0.000009286846,0.00002243118],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002755266,0.00002282564,0.00008113348,0.00002523029,0.000004298072,0.00008386715,0.000006528837,0.00001976468,0.9984142,0.0001632831,0.00007701251,0.0008263052],"study_design_scores_gemma":[0.00005533914,0.0001602099,0.01088513,0.000007386098,0.00001712769,0.0006622511,0.00002269667,0.00057308,0.9828037,0.00008142383,0.004724573,0.000007076765],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.987664,0.002896585,0.004121657,0.0002603585,0.00006282165,0.00003516026,0.0009195681,0.00004972755,0.003990009],"genre_scores_gemma":[0.9721279,0.002357571,0.002970698,0.0001509255,0.00003177715,0.0001087838,0.003349939,0.00003811819,0.01886428],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005891538,"threshold_uncertainty_score":0.01970911,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01740782400061147,"score_gpt":0.2504545764307878,"score_spread":0.2330467524301764,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}