{"id":"W2072736827","doi":"10.1016/s0006-2952(01)00742-0","title":"Sequestration and phosphorylation of the prostaglandin E2 EP4 receptor: dependence on the C-terminal tail33Abbreviations: PGE2, prostaglandin E2, GRK, G-protein coupled receptor kinase; PKA, protein kinase A; PKC, protein kinase C; PCR, polymerase chain reaction; DMEM, Dulbecco’s modified Eagle’s medium; PAS, protein A Sepharose; ECL, electrochemiluminescence; BCA, 2-bicinchoninic acid; IR, immunoreactivity; HBSS, Hanks’ buffered salt solution; TBS, Tris-buffered saline; cAMP, cyclic AMP; PMA, phorbol 12-myristate 13 acetate; and β2-AR, β2-adrenergic receptor.","year":2001,"lang":"en","type":"article","venue":"Biochemical Pharmacology","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":false,"ca_institutions":"TransCanada (Canada)","funders":"","keywords":"Prostaglandin E2 receptor; Forskolin; Protein kinase C; Protein kinase A; Homologous desensitization; Phosphorylation; Adenylyl cyclase; Cell biology; Receptor; Chemistry; Biology; Prostaglandin E2; Agonist; Molecular biology; Signal transduction; Biochemistry; Endocrinology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.001635247,0.001234067,0.001075436,0.000259573,0.0009181824,0.0001861569,0.0007772021,0.001077212,0.0003801053],"category_scores_gemma":[0.0007184962,0.0009745224,0.000350328,0.0007446478,0.0008221986,0.0001408121,0.0003624966,0.001306197,0.0000197882],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004503492,"about_ca_system_score_gemma":0.0006959856,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007869876,"about_ca_topic_score_gemma":0.0001417094,"domain_scores_codex":[0.9928129,0.001327976,0.001664239,0.001925732,0.0009187962,0.001350301],"domain_scores_gemma":[0.9962376,0.0001549197,0.001594687,0.001008291,0.0005084433,0.0004960646],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.006732993,0.0008549534,0.0001907992,0.0002569098,0.0002565191,0.0000148984,0.0002618996,0.00001023941,0.9887977,0.0002095601,0.001601752,0.0008118281],"study_design_scores_gemma":[0.005423738,0.001306216,0.00022825,0.0004298661,0.0001987601,0.00005882964,0.0001193034,0.0008529502,0.9879317,0.0002459049,0.002125843,0.001078656],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9831024,0.004303997,0.0007694333,0.00242638,0.0003687361,0.008546681,0.0003349425,0.0001199765,0.00002747889],"genre_scores_gemma":[0.989539,0.001034033,0.0007045047,0.0002616404,0.000613083,0.004316591,0.001812976,0.0001547654,0.001563425],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006436607,"threshold_uncertainty_score":0.9992705,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01740782400061147,"score_gpt":0.2504545764307878,"score_spread":0.2330467524301764,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}