{"id":"W2073352647","doi":"10.3791/50523","title":"Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate","year":2013,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Parkinson's Disease Mechanisms and Treatments","field":"Medicine","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Canadian Nuclear Safety Commission; Vlaamse regering; Bundesamt für Strahlenschutz; Koning Boudewijnstichting; KU Leuven; Fonds Wetenschappelijk Onderzoek","keywords":"Immunoprecipitation; Phosphorylation; Kinase; Serine; Protein kinase domain; LRRK2; Biochemistry; Blot; Cell biology; Ankyrin repeat; Biology; Molecular biology; Chemistry; Gene; Mutation; Mutant","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001228085,0.0001949953,0.0006561582,0.0001789641,0.00005087972,0.00002193955,0.00006108869,0.00004606861,0.0002211017],"category_scores_gemma":[0.00004172571,0.00012513,0.000101543,0.0001476511,0.00005339869,0.0002654615,0.00002758747,0.00009759574,0.000005719252],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005427759,"about_ca_system_score_gemma":0.0000802307,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001094135,"about_ca_topic_score_gemma":3.909153e-7,"domain_scores_codex":[0.9987142,0.00005488841,0.0004377965,0.0001692005,0.0004265301,0.0001974009],"domain_scores_gemma":[0.9987071,0.00003505142,0.0005068095,0.0001582466,0.000319507,0.0002732884],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.006143682,0.004256477,0.06495097,0.0001436442,0.006171956,0.0005850242,0.002581136,0.000007763979,0.9037917,0.000313771,0.0004741956,0.01057966],"study_design_scores_gemma":[0.01416784,0.001997856,0.03072477,0.0003905421,0.0008990703,0.0003410087,0.0008829218,0.00005136792,0.9496405,0.00008759116,0.0006720345,0.0001445403],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9835894,0.01548846,0.0001833488,0.00006258552,0.00008934097,0.0003035111,0.000004659441,0.000009949811,0.0002687173],"genre_scores_gemma":[0.9879011,0.001171491,0.01047144,0.0001227256,0.00006244889,0.00001488754,0.000003428027,0.00002790544,0.0002245661],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04584874,"threshold_uncertainty_score":0.5102655,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02259278998897,"score_gpt":0.3692498776781633,"score_spread":0.3466570876891933,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}