{"id":"W2074194695","doi":"10.1371/journal.pone.0078749","title":"The Critical Role of DNA Extraction for Detection of Mycobacteria in Tissues","year":2013,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Mycobacterium research and diagnosis","field":"Medicine","cited_by":38,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University; McGill University Health Centre","funders":"Canadian Institutes of Health Research; McGill University Health Centre; McGill University","keywords":"DNA extraction; DNA; Isoamyl alcohol; Biology; Nucleic acid; Polymerase chain reaction; genomic DNA; Paratuberculosis; Restriction digest; Bacteria; Chromatography; Microbiology; Molecular biology; Mycobacterium; Restriction enzyme; Chemistry; Biochemistry; Gene; Genetics; Alcohol","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001728788,0.00004656472,0.0001636833,0.00005815362,0.00002802999,0.00001035581,0.00003952842,0.00005026943,0.0001960462],"category_scores_gemma":[0.001401992,0.0000338071,0.00003396414,0.00007707618,0.00005466744,0.00009652089,0.0000140416,0.00007285765,0.00002163328],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002338647,"about_ca_system_score_gemma":0.00002232691,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002279575,"about_ca_topic_score_gemma":0.00006119275,"domain_scores_codex":[0.9993282,0.00003169904,0.0001959467,0.00008389536,0.0001914072,0.0001688803],"domain_scores_gemma":[0.998966,0.0005631585,0.00003821908,0.0001382322,0.0002330397,0.00006130972],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002537846,0.0007212667,0.01122817,0.0002484366,0.00003337231,3.144654e-7,0.00005749115,1.833174e-8,0.9808081,0.0001037849,0.0000505004,0.006494812],"study_design_scores_gemma":[0.000333252,0.0004907526,0.1838162,0.0001455977,0.00003553362,8.792239e-7,0.0001273862,0.0002038857,0.8140736,0.0005743605,0.0001726214,0.00002584041],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9979951,0.0002408666,0.00002943674,0.0008309908,0.00002480644,0.0005383635,0.000007823718,0.0000094303,0.0003231963],"genre_scores_gemma":[0.9988357,0.0001786286,0.0005365982,0.00001249865,0.00009931059,0.0002344438,0.000005465233,0.000007895849,0.00008945064],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1725881,"threshold_uncertainty_score":0.2146569,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03679633708976249,"score_gpt":0.3057079431830032,"score_spread":0.2689116060932407,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}