{"id":"W2074794440","doi":"10.1006/prep.1999.1180","title":"Membrane-Bound Human 3β-Hydroxysteroid Dehydrogenase: Overexpression with His-Tag Using a Baculovirus System and Single-Step Purification","year":2000,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Hormonal Regulation and Hypertension","field":"Medicine","cited_by":12,"is_retracted":false,"has_abstract":false,"ca_institutions":"Medical Council of Canada; Université Laval","funders":"Medical Research Council; Canadian Space Agency","keywords":"Sf9; Affinity chromatography; Enzyme; Western blot; Dehydrogenase; Membrane; Chemistry; Biochemistry; Chromatography; Recombinant DNA; Molecular biology; Biology; Spodoptera; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002858051,0.0003112869,0.0003888533,0.0001875712,0.0005762442,0.0001284251,0.00008009005,0.0002464676,0.0001823846],"category_scores_gemma":[0.00002301454,0.0002338375,0.00005243417,0.0002046956,0.0001380171,0.0003623865,0.00002564782,0.0002054549,0.00002252473],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001233857,"about_ca_system_score_gemma":0.00005197824,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007054367,"about_ca_topic_score_gemma":0.000003418377,"domain_scores_codex":[0.9978716,0.0001691814,0.0004932359,0.0006488129,0.0005392978,0.0002779014],"domain_scores_gemma":[0.9987561,0.00002565118,0.0002555364,0.0004883949,0.0001554153,0.0003188675],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006073253,0.0001641633,0.0002249639,0.0003380533,0.00001816682,0.00001390231,0.0002564859,0.00002027394,0.9832807,0.0002920977,0.00003873352,0.01474518],"study_design_scores_gemma":[0.003874606,0.0004761818,0.005422834,0.003347906,0.0001597201,0.0006841656,0.0003671134,0.02199782,0.9447959,0.00003467455,0.01830572,0.000533328],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9943223,0.001146681,0.0007985017,0.0002711556,0.00001692444,0.001583482,0.000009105839,0.0001671135,0.001684759],"genre_scores_gemma":[0.9937377,0.00006413593,0.004362432,0.00008384832,0.0001186208,0.0001195758,0.0001303972,0.00005388122,0.001329418],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03848472,"threshold_uncertainty_score":0.9535615,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02985546108300388,"score_gpt":0.2567353661576838,"score_spread":0.2268799050746799,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}