{"id":"W2075068616","doi":"10.1093/protein/gzg091","title":"Precise and efficient cleavage of recombinant fusion proteins using mammalian aspartic proteases","year":2003,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"Sembiosys Genetics (Canada)","funders":"","keywords":"Recombinant DNA; Fusion protein; Tobacco etch virus; Biochemistry; Thioredoxin; Escherichia coli; Cleavage (geology); Enteropeptidase; Chemistry; FLAG-tag; Myc-tag; Cystatin; Protein tag; Protease; Proteases; Tandem affinity purification; Peptide; Target peptide; Biology; Affinity chromatography; Enzyme; Cystatin C; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001096179,0.0007598497,0.0007514489,0.0003913609,0.0002178138,0.0006262154,0.000432346,0.0006151954,0.000436854],"category_scores_gemma":[0.0006489837,0.0004126303,0.0006030647,0.0005144434,0.0003972455,0.0004814629,0.0005348812,0.001197977,0.0007810247],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003479091,"about_ca_system_score_gemma":0.0003634206,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002449592,"about_ca_topic_score_gemma":0.0004423376,"domain_scores_codex":[0.9990048,0.0001939721,0.0001838878,0.0001834222,0.0003489773,0.00008499774],"domain_scores_gemma":[0.9995711,0.0001069756,0.0001074541,0.00007825725,0.00009350494,0.00004253994],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001852056,0.00000769553,0.00004747755,0.00004216069,0.000004952915,0.00004304134,0.000009070404,0.00004260445,0.9982097,0.00009210366,0.00003166794,0.001450964],"study_design_scores_gemma":[0.00001135871,0.00008534749,0.0005938381,0.000005314942,0.00001274276,0.0005042963,0.00001122042,0.0004618918,0.9936599,0.00008711536,0.004560595,0.000006391595],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5649703,0.007723199,0.4215355,0.0004229405,0.0001911306,0.0006701088,0.001178907,0.0006508571,0.00265722],"genre_scores_gemma":[0.6152703,0.01048485,0.3609606,0.000270462,0.00008725009,0.0003655295,0.005745416,0.0002342315,0.006581381],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001096179,"threshold_uncertainty_score":0.005797207,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02809522144840556,"score_gpt":0.2580447007734568,"score_spread":0.2299494793250512,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}