{"id":"W2075068616","doi":"10.1093/protein/gzg091","title":"Precise and efficient cleavage of recombinant fusion proteins using mammalian aspartic proteases","year":2003,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":17,"is_retracted":false,"has_abstract":true,"ca_institutions":"Sembiosys Genetics (Canada)","funders":"","keywords":"Recombinant DNA; Fusion protein; Tobacco etch virus; Biochemistry; Thioredoxin; Escherichia coli; Cleavage (geology); Enteropeptidase; Chemistry; FLAG-tag; Myc-tag; Cystatin; Protein tag; Protease; Proteases; Tandem affinity purification; Peptide; Target peptide; Biology; Affinity chromatography; Enzyme; Cystatin C; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003747711,0.0001336616,0.0002018364,0.0001444032,0.00009055696,0.00001743188,0.00002136248,0.00006675868,0.00001336433],"category_scores_gemma":[0.0001681728,0.0001108825,0.0000307103,0.0002181333,0.00003760766,0.00003968531,0.00001650269,0.0001547857,0.000001190842],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003539624,"about_ca_system_score_gemma":0.00005330381,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006824904,"about_ca_topic_score_gemma":7.871456e-7,"domain_scores_codex":[0.9990953,0.00007616297,0.000195707,0.0002078005,0.0001931557,0.00023192],"domain_scores_gemma":[0.9996653,0.00003127069,0.00004518356,0.00006374551,0.00006445227,0.0001300932],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003806676,0.0001613495,0.0009346246,0.0007181169,0.00002629391,0.000009334155,0.0001057001,0.001747444,0.9926796,0.0005188639,0.0000102651,0.002707762],"study_design_scores_gemma":[0.0007087843,0.00096149,0.002950834,0.0004044921,0.00003282475,0.0001847808,0.00001220437,0.1670718,0.8270501,0.00006483386,0.0004122684,0.0001455637],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9226984,0.0005868028,0.0747135,0.00005423521,0.00003387636,0.001836622,5.714751e-7,0.00003712754,0.00003888493],"genre_scores_gemma":[0.9662929,0.00004183309,0.03316965,0.000003516217,0.00002422226,0.0001002192,9.809976e-7,0.00001861318,0.0003480635],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1656294,"threshold_uncertainty_score":0.4521659,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02809522144840556,"score_gpt":0.2580447007734568,"score_spread":0.2299494793250512,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}