{"id":"W2075611273","doi":"10.1016/j.pep.2006.02.016","title":"Long stretches of sequential and identical serine or alanine codons are compatible with an efficient full-length protein expression in Escherichia coli","year":2006,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Bacteriophages and microbial interactions","field":"Environmental Science","cited_by":0,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Institute of Genetics","keywords":"Escherichia coli; Fusion protein; Biology; Recombinant DNA; Gene; Biochemistry; Molecular biology; Serine; FLAG-tag; Affinity chromatography; Enzyme","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004973277,0.0006552918,0.0006600743,0.0004034502,0.0003522866,0.0009722938,0.0003321882,0.0005646516,0.0008624442],"category_scores_gemma":[0.0006252939,0.0006522755,0.000635204,0.0005808865,0.0005683522,0.000422771,0.000481745,0.001237525,0.001091521],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005011664,"about_ca_system_score_gemma":0.0006145879,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009104555,"about_ca_topic_score_gemma":0.001732836,"domain_scores_codex":[0.9995523,0.0001117153,0.00006492286,0.00005807406,0.0001261724,0.00008678861],"domain_scores_gemma":[0.9991958,0.0002816035,0.0001471028,0.0001236787,0.00007202888,0.0001798526],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001120203,0.00003259672,0.00009117465,0.0000290241,0.000006689193,0.00003734741,0.0000180288,0.00007612306,0.9989313,0.0001202889,0.00003481361,0.0005105972],"study_design_scores_gemma":[0.0000155232,0.0001048451,0.001154354,0.000008422675,0.00001951135,0.0001452091,0.0000240978,0.0005368834,0.9967026,0.00007115419,0.001208491,0.000008927615],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9832805,0.0005645804,0.01388334,0.0001933091,0.00006971571,0.00007783226,0.0008558488,0.0002215218,0.0008531794],"genre_scores_gemma":[0.9742587,0.0005570849,0.01783735,0.0001646576,0.00004132015,0.00007215831,0.003134667,0.0002489962,0.003685043],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009722938,"threshold_uncertainty_score":0.003636241,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01291250336241245,"score_gpt":0.2425076537545353,"score_spread":0.2295951503921228,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}